Estrada-Franco J G, Lanzaro G C, Ma M C, Walker-Abbey A, Romans P, Galvan-Sanchez C, Cespedes J L, Vargas-Sagarnaga R, Laughinghouse A, Columbus I
Department of Entomology, University of Maryland, College Park.
Am J Trop Med Hyg. 1993 Dec;49(6):735-45. doi: 10.4269/ajtmh.1993.49.735.
Enzyme electrophoresis and restriction fragment length polymorphism (RFLP) analysis of Anopheles pseudopunctipennis sensu lato from nine isolated populations in neotropical America confirmed previous observations that it constitutes a species complex. Electrophoretic studies showed fixed differences at two enzyme loci, glycerol dehydrogenase (Gcd) and phosphoglucomutase (Pgm), suggesting limited or no gene flow between populations from Mexico and South America. In addition, analysis of genetic distance showed two distinctive clusters, one from Mexico and the other from South America, separated at a Nei's distance level of 0.13, a value consistent in magnitude with that of other anopheline sibling species. The RFLP analysis revealed the presence of a ribosomal DNA fragment in Mexican strains that was absent in strains from South America. Two species have been identified through these studies, one provisionally named An. pseudopunctipennis A, a species from central Mexico, and the other An. pseudopunctipennis B, for the species found in the interAndean valleys and Andean slopes in regions of Peru and Bolivia. This research provides information required to elucidate the status of the different species of the An. pseudopunctipennis complex as vectors of malaria in the Americas.
对新热带界美洲九个孤立种群的伪点按蚊复合种进行酶电泳和限制性片段长度多态性（RFLP）分析，证实了之前的观察结果，即它构成一个复合种。电泳研究显示在两个酶位点，即甘油脱氢酶（Gcd）和磷酸葡萄糖变位酶（Pgm）上存在固定差异，这表明墨西哥和南美洲种群之间的基因流动有限或不存在。此外，遗传距离分析显示出两个不同的聚类，一个来自墨西哥，另一个来自南美洲，在Nei氏距离水平为0.13时分开，该值在大小上与其他按蚊姐妹种的值一致。RFLP分析揭示了墨西哥菌株中存在一个核糖体DNA片段，而南美洲菌株中不存在。通过这些研究已鉴定出两个物种，一个暂定为伪点按蚊A，是来自墨西哥中部的一个物种，另一个为伪点按蚊B，是在秘鲁和玻利维亚地区安第斯山脉间山谷和安第斯山坡发现的物种。这项研究提供了阐明伪点按蚊复合种不同物种作为美洲疟疾传播媒介的地位所需的信息。
