一组多态性牛、羊和山羊微卫星标记

A panel of polymorphic bovine, ovine and caprine microsatellite markers.

作者信息

Kemp S J, Hishida O, Wambugu J, Rink A, Longeri M L, Ma R Z, Da Y, Lewin H A, Barendse W, Teale A J

机构信息

International Laboratory for Research on Animal Diseases, Nairobi, Kenya.

出版信息

Anim Genet. 1995 Oct;26(5):299-306. doi: 10.1111/j.1365-2052.1995.tb02663.x.

DOI:10.1111/j.1365-2052.1995.tb02663.x

PMID:7486246

Abstract

A panel of 81 new polymorphic bovine microsatellite markers is described, together with further information on a previously reported group of 16 markers. The mean polymorphism information content of the 97 markers determined in 20 cattle was 0.66. Seventy-three of these markers have been assigned to chromosomes by either linkage analysis or use of hybrid cell panels. Thirty-nine of the markers were polymorphic in sheep, and 32 were polymorphic in goat. This study identified a set of 18 robust markers that were polymorphic in all three species and that covered 14 bovine chromosomes. This provides a single group of markers, which would be suited to genetic distance analysis and parentage control in cattle, sheep and goat.

摘要

本文描述了一组81个新的多态性牛微卫星标记，并给出了先前报道的16个标记组的更多信息。在20头牛中测定的97个标记的平均多态性信息含量为0.66。通过连锁分析或使用杂交细胞板，已将其中73个标记定位到染色体上。其中39个标记在绵羊中具有多态性，32个标记在山羊中具有多态性。本研究鉴定出一组18个稳健的标记，这些标记在所有三个物种中均具有多态性，并覆盖了14条牛染色体。这提供了一组单一的标记，适用于牛、绵羊和山羊的遗传距离分析和亲子鉴定。