将五个多态性绵羊微卫星定位到牛的同线群

Assignment of five polymorphic ovine microsatellites to bovine syntenic groups.

作者信息

Dietz A B, Womack J E, Swarbrick P A, Crawford A M

机构信息

Texas A&M University, College Station.

出版信息

Anim Genet. 1993 Dec;24(6):433-6. doi: 10.1111/j.1365-2052.1993.tb00357.x.

DOI:10.1111/j.1365-2052.1993.tb00357.x

PMID:7903848

Abstract

A panel of bovine somatic cell hybrids was used to map ovine microsatellites. Five of seven microsatellites were assigned to five bovine syntenic groups. These microsatellites were designated D5S10 (MAF23), D1S4 (MAF46), D13S1 (MAF18), D4S3 (MAF50), and DXS2 (MAF45), mapped to syntenic groups U3 (chromosome 5), U10 (chromosome 1), U11, U13, and the X chromosome, respectively. Two remaining sheep microsatellites amplified rodent DNA in the hybrid somatic cell panel, and were not assigned to bovine syntenic groups. Assignment of ovine-derived microsatellites to bovine syntenic groups provides additional evidence of the usefulness of microsatellites for mapping closely related species. The use of ovine and bovine microsatellites will aid in development of comparative genomic maps for these two species.

摘要

利用一组牛体细胞杂种来绘制绵羊微卫星图谱。七个微卫星中的五个被定位到五个牛同线群。这些微卫星分别被命名为D5S10（MAF23）、D1S4（MAF46）、D13S1（MAF18）、D4S3（MAF50）和DXS2（MAF45），它们分别被定位到同线群U3（5号染色体）、U10（1号染色体）、U11、U13和X染色体上。其余两个绵羊微卫星在杂种体细胞组中扩增出啮齿动物DNA，未被定位到牛同线群。将绵羊来源的微卫星定位到牛同线群，为微卫星在绘制近缘物种图谱中的实用性提供了更多证据。绵羊和牛微卫星的使用将有助于这两个物种比较基因组图谱的绘制。