[使用PRINS技术直接分析人类精子中二体的频率]
[Direct analysis of the frequency of disomy in human sperm using the PRINS technique].
作者信息
Quenesson I, Girardet A, Coignet L, Andréo B, Lefort G, Charlieu J P, Pellestor F
机构信息
CNRS UPR 9008, BP 5051, Montpellier, France.
出版信息
Ann Genet. 1995;38(2):85-9.
The PRINS method allows a rapid and specific detection of human chromosomes in situ. We have adapted the PRINS protocol to human sperm. Estimates of disomy have thus been performed for chromosomes 9, 12, 16 and 21 by using alpha-satellite DNA specific primers. The frequencies of disomy ranged from 0.27% to 0.31%. No significant difference was found. These data agree with the hypothesis of an equal distribution of non-disjunctions among chromosomes in male meiosis.
PRINS方法可在原位快速、特异性地检测人类染色体。我们已将PRINS方案应用于人类精子。因此，通过使用α-卫星DNA特异性引物对9号、12号、16号和21号染色体进行了二体性估计。二体性频率在0.27%至0.31%之间。未发现显著差异。这些数据与男性减数分裂中染色体不分离均等分布的假设相符。
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