Brück W, Porada P, Poser S, Rieckmann P, Hanefeld F, Kretzschmar H A, Lassmann H
Department of Neuropathology, University of Göttingen, Germany.
Ann Neurol. 1995 Nov;38(5):788-96. doi: 10.1002/ana.410380514.
Monocyte/macrophage differentiation was studied in biopsy samples of multiple sclerosis (MS) lesions obtained in the early course of the disease. Macrophages were identified by immunocytochemistry using a panel of antibodies recognizing different macrophage-activation antigens. The number of cells stained with each antibody was related to the demyelinating activity of the lesions as detected by the presence of myelin degradation products. The pan-macrophage marker Ki-M1P revealed the highest numbers of macrophages in early and late active lesions. Lower numbers were encountered in inactive, demyelinated, or remyelinated lesions. The acute stage inflammatory macrophage markers MRP14 and 27E10 were expressed in either only early active (MRP14) or early and late active (27E10) lesions, thus allowing the identification of actively demyelinating lesions. The chronic stage inflammatory macrophage marker 25F9, in contrast, showed increasing expression with decreasing lesional activity. These findings indicate a differentiated pattern of macrophage activation in MS lesions and allow the staging of demyelinating lesions in routinely fixed and paraffin-embedded tissue.
在疾病早期获取的多发性硬化症(MS)病变活检样本中研究了单核细胞/巨噬细胞分化情况。使用一组识别不同巨噬细胞活化抗原的抗体,通过免疫细胞化学鉴定巨噬细胞。用每种抗体染色的细胞数量与通过髓磷脂降解产物的存在检测到的病变脱髓鞘活性相关。全巨噬细胞标志物Ki-M1P显示在早期和晚期活动病变中巨噬细胞数量最多。在非活动、脱髓鞘或再髓鞘化病变中数量较少。急性期炎症巨噬细胞标志物MRP14和27E10仅在早期活动(MRP14)或早期和晚期活动(27E10)病变中表达,从而有助于识别正在进行脱髓鞘的病变。相比之下,慢性期炎症巨噬细胞标志物25F9随着病变活动度降低而表达增加。这些发现表明MS病变中巨噬细胞活化存在分化模式,并有助于在常规固定和石蜡包埋组织中对脱髓鞘病变进行分期。