编码青霉素结合蛋白3的铜绿假单胞菌pbpB基因的克隆与特性分析
Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3.
作者信息
Liao X, Hancock R E
机构信息
Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada.
出版信息
Antimicrob Agents Chemother. 1995 Aug;39(8):1871-4. doi: 10.1128/AAC.39.8.1871.
Abstract
Clones containing the pbpB gene which encodes penicillin-binding protein (PBP) 3 of Pseudomonas aeruginosa were detected by hybridization by PCR amplification with primers based on the conserved sequences of high-molecular-weight PBPs. The translated amino acid sequence demonstrated 45% identity and had a total of 66% conserved amino acids relative to the Escherichia coli PBP3. The pbpB gene was located upstream of a gene homologous to the E. coli murE gene, which encodes uridine diphosphate-N-acetyl muramic acid-tripeptide synthetase. The overexpressed pbpB gene product reacted with 3H-penicillin G and had an apparent molecular weight of 60,000.
摘要
通过基于高分子量青霉素结合蛋白（PBP）保守序列设计的引物进行PCR扩增杂交，检测到含有编码铜绿假单胞菌青霉素结合蛋白（PBP）3的pbpB基因的克隆。翻译后的氨基酸序列与大肠杆菌PBP3相比，显示出45%的同一性，并且共有66%的保守氨基酸。pbpB基因位于与大肠杆菌murE基因同源的基因上游，murE基因编码尿苷二磷酸-N-乙酰胞壁酸-三肽合成酶。过表达的pbpB基因产物与3H-青霉素G发生反应，其表观分子量为60,000。
相似文献
1
Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3.
Antimicrob Agents Chemother. 1995 Aug;39(8):1871-4. doi: 10.1128/AAC.39.8.1871.
2
Identification of a penicillin-binding protein 3 homolog, PBP3x, in Pseudomonas aeruginosa: gene cloning and growth phase-dependent expression.
J Bacteriol. 1997 Mar;179(5):1490-6. doi: 10.1128/jb.179.5.1490-1496.1997.
3
Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis.
J Bacteriol. 1993 Dec;175(23):7604-16. doi: 10.1128/jb.175.23.7604-7616.1993.
4
The Bacillus subtilis spoVD gene encodes a mother-cell-specific penicillin-binding protein required for spore morphogenesis.
J Mol Biol. 1994 Jan 7;235(1):209-20. doi: 10.1016/s0022-2836(05)80027-0.
5
Comparative analysis of Pseudomonas aeruginosa penicillin-binding protein 7 in the context of its membership in the family of low-molecular-mass PBPs.
Microbiology (Reading). 1998 Apr;144 ( Pt 4):975-983. doi: 10.1099/00221287-144-4-975.
6
A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.
J Bacteriol. 1996 Apr;178(8):2343-50. doi: 10.1128/jb.178.8.2343-2350.1996.
7
Sequence of the ponA gene and characterization of the penicillin-binding protein 1A of Pseudomonas aeruginosa PAO1.
Gene. 1997 Oct 15;199(1-2):49-56. doi: 10.1016/s0378-1119(97)00345-4.
8
Identification of a new mutation in Escherichia coli that suppresses a pbpB (Ts) phenotype in the presence of penicillin-binding protein 1B.
FEMS Microbiol Lett. 1991 Nov 1;68(1):7-13. doi: 10.1016/0378-1097(91)90386-o.
9
Penicillin-binding proteins in Leptospira interrogans.
Antimicrob Agents Chemother. 2001 Mar;45(3):870-7. doi: 10.1128/AAC.45.3.870-877.2001.
10
pbpB, a gene coding for a putative penicillin-binding protein, is required for aerobic nitrogen fixation in the cyanobacterium Anabaena sp. strain PCC7120.
J Bacteriol. 2001 Jan;183(2):628-36. doi: 10.1128/JB.183.2.628-636.2001.
引用本文的文献
1
High potency of sequential therapy with only β-lactam antibiotics.
Elife. 2021 Jul 28;10:e68876. doi: 10.7554/eLife.68876.
2
Peptidoglycomics reveals compositional changes in peptidoglycan between biofilm- and planktonic-derived .
J Biol Chem. 2020 Jan 10;295(2):504-516. doi: 10.1074/jbc.RA119.010505. Epub 2019 Nov 26.
3
Pan-genome dynamics of Pseudomonas gene complements enriched across hexachlorocyclohexane dumpsite.
BMC Genomics. 2015 Apr 18;16(1):313. doi: 10.1186/s12864-015-1488-2.
4
Genome analysis of a transmissible lineage of pseudomonas aeruginosa reveals pathoadaptive mutations and distinct evolutionary paths of hypermutators.
PLoS Genet. 2013;9(9):e1003741. doi: 10.1371/journal.pgen.1003741. Epub 2013 Sep 5.
5
Tracking down antibiotic-resistant Pseudomonas aeruginosa isolates in a wastewater network.
PLoS One. 2012;7(12):e49300. doi: 10.1371/journal.pone.0049300. Epub 2012 Dec 19.
6
Overproduction of penicillin-binding protein 2 and its inactive variants causes morphological changes and lysis in Escherichia coli.
J Bacteriol. 2007 Jul;189(14):4975-83. doi: 10.1128/JB.00207-07. Epub 2007 May 18.
7
Identification of quorum-sensing-regulated genes of Burkholderia cepacia.
J Bacteriol. 2003 Nov;185(21):6456-62. doi: 10.1128/JB.185.21.6456-6462.2003.
8
Morphological changes and lysis induced by beta-lactams associated with the characteristic profiles of affinities of penicillin-binding proteins in actinobacillus pleuropneumoniae.
Antimicrob Agents Chemother. 2000 Jun;44(6):1518-23. doi: 10.1128/AAC.44.6.1518-1523.2000.
9
Visualization of penicillin-binding proteins during sporulation of Streptomyces griseus.
J Bacteriol. 1998 Apr;180(8):2125-32. doi: 10.1128/JB.180.8.2125-2132.1998.
10
Susceptibility to beta-lactam antibiotics of Pseudomonas aeruginosa overproducing penicillin-binding protein 3.
Antimicrob Agents Chemother. 1997 May;41(5):1158-61. doi: 10.1128/AAC.41.5.1158.
本文引用的文献
1
Cloning and sequencing of the low-affinity penicillin-binding protein 3r-encoding gene of Enterococcus hirae S185: modular design and structural organization of the protein.
J Bacteriol. 1993 May;175(10):2844-52. doi: 10.1128/jb.175.10.2844-2852.1993.
2
pBBR1MCS: a broad-host-range cloning vector.
Biotechniques. 1994 May;16(5):800-2.
3
beta-Lactam-resistant Pseudomonas aeruginosa with modified penicillin-binding proteins emerging during cystic fibrosis treatment.
Antimicrob Agents Chemother. 1981 May;19(5):705-11. doi: 10.1128/AAC.19.5.705.
4
Penicillin-binding proteins and the future of beta-lactam antibiotics. The Seventh Fleming Lecture.
J Gen Microbiol. 1983 May;129(5):1247-60. doi: 10.1099/00221287-129-5-1247.
5
Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine.
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1133-41. doi: 10.1073/pnas.54.4.1133.
6
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
J Mol Biol. 1986 May 5;189(1):113-30. doi: 10.1016/0022-2836(86)90385-2.
7
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8. doi: 10.1073/pnas.82.4.1074.
8
In vitro evaluation of E1040, a new cephalosporin with potent antipseudomonal activity.
Antimicrob Agents Chemother. 1988 May;32(5):693-701. doi: 10.1128/AAC.32.5.693.
9
Penicillin-binding proteins of gram-negative bacteria.
Rev Infect Dis. 1988 Jul-Aug;10(4):699-711. doi: 10.1093/clinids/10.4.699.
10
Lipid modification of Escherichia coli penicillin-binding protein 3.
J Bacteriol. 1988 Nov;170(11):5392-5. doi: 10.1128/jb.170.11.5392-5395.1988.
Zotero 插件