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Cloning and sequencing of the low-affinity penicillin-binding protein 3r-encoding gene of Enterococcus hirae S185: modular design and structural organization of the protein.平肠球菌S185低亲和力青霉素结合蛋白3r编码基因的克隆与测序：该蛋白的模块化设计与结构组织
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pBBR1MCS: a broad-host-range cloning vector.pBBR1MCS：一种广宿主范围的克隆载体。
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beta-Lactam-resistant Pseudomonas aeruginosa with modified penicillin-binding proteins emerging during cystic fibrosis treatment.在囊性纤维化治疗期间出现的具有修饰青霉素结合蛋白的β-内酰胺耐药铜绿假单胞菌。
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Penicillin-binding proteins and the future of beta-lactam antibiotics. The Seventh Fleming Lecture.青霉素结合蛋白与β-内酰胺类抗生素的未来。第七届弗莱明讲座。
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Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine.青霉素的作用机制：基于其与酰基-D-丙氨酰-D-丙氨酸结构相似性的一种假说
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A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.一种用于特异性基因的可控专一性表达的噬菌体T7 RNA聚合酶/启动子系统。
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In vitro evaluation of E1040, a new cephalosporin with potent antipseudomonal activity.新型具有强效抗假单胞菌活性的头孢菌素E1040的体外评价
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Penicillin-binding proteins of gram-negative bacteria.革兰氏阴性菌的青霉素结合蛋白
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Lipid modification of Escherichia coli penicillin-binding protein 3.大肠杆菌青霉素结合蛋白3的脂质修饰
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编码青霉素结合蛋白3的铜绿假单胞菌pbpB基因的克隆与特性分析

Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3.

作者信息

Liao X, Hancock R E

机构信息

Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada.

出版信息

Antimicrob Agents Chemother. 1995 Aug;39(8):1871-4. doi: 10.1128/AAC.39.8.1871.

DOI:10.1128/AAC.39.8.1871
PMID:7486937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC162844/
Abstract

Clones containing the pbpB gene which encodes penicillin-binding protein (PBP) 3 of Pseudomonas aeruginosa were detected by hybridization by PCR amplification with primers based on the conserved sequences of high-molecular-weight PBPs. The translated amino acid sequence demonstrated 45% identity and had a total of 66% conserved amino acids relative to the Escherichia coli PBP3. The pbpB gene was located upstream of a gene homologous to the E. coli murE gene, which encodes uridine diphosphate-N-acetyl muramic acid-tripeptide synthetase. The overexpressed pbpB gene product reacted with 3H-penicillin G and had an apparent molecular weight of 60,000.

摘要

通过基于高分子量青霉素结合蛋白（PBP）保守序列设计的引物进行PCR扩增杂交，检测到含有编码铜绿假单胞菌青霉素结合蛋白（PBP）3的pbpB基因的克隆。翻译后的氨基酸序列与大肠杆菌PBP3相比，显示出45%的同一性，并且共有66%的保守氨基酸。pbpB基因位于与大肠杆菌murE基因同源的基因上游，murE基因编码尿苷二磷酸-N-乙酰胞壁酸-三肽合成酶。过表达的pbpB基因产物与3H-青霉素G发生反应，其表观分子量为60,000。