一个包含枯草芽孢杆菌中必需细胞分裂基因pbpB的复杂四基因操纵子。
A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.
作者信息
Daniel R A, Williams A M, Errington J
机构信息
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
出版信息
J Bacteriol. 1996 Apr;178(8):2343-50. doi: 10.1128/jb.178.8.2343-2350.1996.
Abstract
We have cloned and sequenced the promoter-proximal region of the Bacillus subtilis operon containing the pbpB gene, encoding essential penicillin-binding protein PBP2B. The first two genes in the operon, designated yllB and yllC, are significantly similar to genes of unknown function similarly positioned upstream of pbpB in Escherichia coli. Both B. subtilis genes are shown to be nonessential. The third B. subtilis gene, yllD, is essential, as is the correspondingly positioned ftsL gene of E. coli. The predicted product of yllD is similar to FtsL in size and distribution of charged residues but is not significantly related in primary amino acid sequence. The major promoter for the cluster lies upstream of the first gene, yllB, but at least one minor promoter lies within the yllC gene. The operon is transcribed throughout growth at a low level.
摘要
我们已经克隆并测序了枯草芽孢杆菌操纵子的启动子近端区域，该操纵子包含编码必需青霉素结合蛋白PBP2B的pbpB基因。该操纵子中的前两个基因，命名为yllB和yllC，与大肠杆菌中位于pbpB上游类似位置的功能未知基因显著相似。已证明枯草芽孢杆菌的这两个基因都不是必需的。枯草芽孢杆菌的第三个基因yllD是必需的，大肠杆菌中相应位置的ftsL基因也是必需的。yllD的预测产物在大小和带电残基分布上与FtsL相似，但在一级氨基酸序列上没有显著相关性。该基因簇的主要启动子位于第一个基因yllB的上游，但至少有一个次要启动子位于yllC基因内。该操纵子在整个生长过程中都以低水平转录。
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