干扰贾第虫包囊和隐孢子虫卵囊检测的藻类鉴定及减轻这种干扰的方法。
Identification of algae which interfere with the detection of Giardia cysts and Cryptosporidium oocysts and a method for alleviating this interference.
作者信息
Rodgers M R, Flanigan D J, Jakubowski W
机构信息
National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268, USA.
出版信息
Appl Environ Microbiol. 1995 Oct;61(10):3759-63. doi: 10.1128/aem.61.10.3759-3763.1995.
Abstract
Fifty-four algal species were tested for cross-reaction in the American Society for Testing and Materials Giardia/Cryptosporidium indirect immunofluorescence assay, and 24 showed some degree of fluorescence. Two species, Navicula minima and Synechococcus elongatus, exhibited a bright apple green fluorescence. The addition of goat serum to the assay mixture blocked the fluorescence of most nontarget organisms tested and also decreased the background fluorescence. Goat serum did not interfere with the fluorescence of Giardia cysts or Cryptosporidium oocysts or the identification of cyst and oocyst internal structures.
摘要
在美国材料与试验协会的贾第虫/隐孢子虫间接免疫荧光检测中，对54种藻类进行了交叉反应测试，其中24种显示出一定程度的荧光。极小舟形藻和聚球藻两种藻类呈现出明亮的苹果绿色荧光。在检测混合物中加入山羊血清可阻断大多数受试非靶标生物的荧光，并降低背景荧光。山羊血清不会干扰贾第虫包囊或隐孢子虫卵囊的荧光，也不会影响包囊和卵囊内部结构的识别。
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