Baldy-Chudzik K, Nowakowska B, Matej H, Kuśnierczyk P
Laboratory of Tissue Immunology, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1994;42(3):227-30.
26 sera from hypersensitized renal patients were analysed for immunoglobulin class of alloantibodies. To assess the participation of IgM and IgG in alloreactivity, sera were treated with dithiothreitol (DTT) or Staphylococcus aureus protein A (SpA), respectively. Treatment with both DTT and SpA was also performed to test the possible contribution of other immunoglobulin classes. Immunological complexes which may mimic alloreactivity were excluded by polyethylene glycol (PEG) treatment. Immunochemically modified sera were tested against a panel of allogeneic B and T lymphocytes. IgG class alloantibodies reactive with B lymphocytes were found two times more frequently than those reactive with T lymphocytes. In 22% of sera only IgM alloantibodies were detected. No contribution of other immunoglobulin classes of immune complexes was seen. Our results show that DTT and SpA treatment are technically simple and reproducible methods of discriminating between IgG and IgM alloantibodies and can be recommended for routine use in clinical analysis of sera from hypersensitized renal patients awaiting kidney graft.
对26份来自致敏肾病患者的血清进行分析，以确定同种抗体的免疫球蛋白类别。为评估IgM和IgG在同种反应性中的作用，血清分别用二硫苏糖醇（DTT）或金黄色葡萄球菌蛋白A（SpA）处理。同时用DTT和SpA处理血清，以检测其他免疫球蛋白类别的可能作用。通过聚乙二醇（PEG）处理排除可能模拟同种反应性的免疫复合物。用一组同种异体B淋巴细胞和T淋巴细胞检测经免疫化学修饰的血清。与B淋巴细胞反应的IgG类同种抗体的发现频率是与T淋巴细胞反应的同种抗体的两倍。在22%的血清中仅检测到IgM同种抗体。未发现其他免疫球蛋白类别或免疫复合物的作用。我们的结果表明，DTT和SpA处理是区分IgG和IgM同种抗体的技术简单且可重复的方法，可推荐用于等待肾移植的致敏肾病患者血清的临床常规分析。
