Baldy-Chudzik K, Nowakowska B, Kuśnierczyk P, Matej H
Laboratory of Tissue Immunology, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1994;42(3):223-6.
We applied K562 erythroblastoma cell line as a substitute for autologous lymphocytes in the absorption of autoantibodies from sera of 26 hypersensitized renal patients exhibiting high degree of cytotoxic reactivity with a panel of lymphocytes from unrelated individuals (panel reactivity, PRA). All of analysed sera contained allo-reactivity before absorption. After removal of autoantibodies, 85% of the sera still contained alloantibodies whereas in 15% of the sera no alloreactivity was detectable. Absorption of high PRA sera with K562 cells and with autologous lymphoblastoid cell lines derived by transformation with Epstein-Barr virus (EBV-LCL) gave substantially the same results. As autoantibodies have reportedly no effect on kidney graft survival, the autoabsorption of high PRA sera may increase the chance of patients in whom only autoantibodies were detected to receive the transplant. Application of K562 cells, instead of autologous lymphocytes/lymphoblasts, is recommended whenever high numbers of patient's cells are not available. It may be particularly suitable for routine clinical laboratories, not equipped and prepared for derivation and propagation of EBV-LCL.
我们应用K562成红细胞瘤细胞系替代自体淋巴细胞，从26例超敏反应性肾病患者的血清中吸收自身抗体，这些患者与一组无关个体的淋巴细胞（群体反应性，PRA）表现出高度的细胞毒性反应。所有分析的血清在吸收前均含有同种异体反应性。去除自身抗体后，85%的血清仍含有同种异体抗体，而15%的血清未检测到同种异体反应性。用K562细胞和经EB病毒转化获得的自体淋巴母细胞系（EBV-LCL）吸收高PRA血清，结果基本相同。据报道，自身抗体对肾移植存活无影响，高PRA血清的自身吸收可能会增加仅检测到自身抗体的患者接受移植的机会。当无法获得大量患者细胞时，建议应用K562细胞而非自体淋巴细胞/淋巴母细胞。它可能特别适用于未配备和准备好进行EBV-LCL衍生和增殖的常规临床实验室。
