Daniluk J, Kandefer-Szerszeń M
Department and Clinic of Gastroenterology, Medical Academy, Lublin, Poland.
Arch Immunol Ther Exp (Warsz). 1994;42(3):239-46.
The effect of acute (single dose) and long-term ethanol treatment of Swiss Albino and C3H/He mice on IFN production stimulated in vitro by NDV, PHA and ConA was examined. Liver organ cultures and spleen cells of mice which received intraperitoneally a single dose (2 g/kg of body weight) of ethanol (II group) or were drinking ethanol solutions instead of water for 4 weeks (III group) or for 8 weeks (IV group), and cells of control mice not treated with ethanol (I group) were cultivated in vitro. Some of the cultures were treated with 0.4% (w/v) ethanol or several IFN inducers NDV-R, PHA and ConA or incubated without any stimulators. The titers of IFN in supernatant fluids were measured. The results showed that cells from mice, which received a single ethanol injection, produced IFN spontaneously and this production was enhanced in the presence of 0.4% ethanol in culture medium. When spleen cell cultures of acute treated mice were induced with PHA and ConA, IFN titers lower than in cells of the control mice were produced. In contrast to these results, liver and spleen cells of mice after long term ethanol consumption produced significantly lower IFN titers than controls after NDV-R induction. Also spleen cells responded poorly both to PHA and ConA induction. These results demonstrate that long-term ethanol treatment can destroy IFN response to viral infection and can impair mitogen-induced IFN production. This suppression of IFN production by long term ethanol was similar in both strains of the mice examined.
研究了急性（单次剂量）和长期乙醇处理对瑞士白化小鼠和C3H/He小鼠的影响，这些小鼠经新城疫病毒（NDV）、植物血凝素（PHA）和刀豆蛋白A（ConA）体外刺激后产生干扰素（IFN）。给小鼠腹腔注射单次剂量（2 g/kg体重）的乙醇（II组），或让小鼠饮用乙醇溶液而非水4周（III组）或8周（IV组），然后培养这些小鼠的肝脏器官培养物和脾细胞，同时培养未用乙醇处理的对照小鼠（I组）的细胞。部分培养物用0.4%（w/v）乙醇、几种IFN诱导剂NDV-R、PHA和ConA处理，或在无任何刺激物的情况下孵育。测定上清液中的IFN滴度。结果显示，单次注射乙醇的小鼠细胞可自发产生IFN，且在培养基中存在0.4%乙醇时，这种产生会增强。当用PHA和ConA诱导急性处理小鼠的脾细胞培养物时，产生的IFN滴度低于对照小鼠细胞。与这些结果相反，长期摄入乙醇的小鼠的肝脏和脾细胞在经NDV-R诱导后产生的IFN滴度明显低于对照。此外，脾细胞对PHA和ConA诱导的反应也很差。这些结果表明，长期乙醇处理可破坏对病毒感染的IFN反应，并损害丝裂原诱导的IFN产生。在所检测的两种小鼠品系中，长期乙醇对IFN产生的这种抑制作用相似。
