Role of bovine albumin in a serum-free culture medium and its application.

Our experiments showed that fatty acids bound to bovine serum albumin V (BSA) played an important role in the growth of cells in primary culture. A serum-free culture medium containing BSA could support continuous growth of various normal and malignant cells from primary culture, and the amount of BSA in the medium could be reduced by supplementation with minute amounts of basic high polymers.