Kar S, Yousem S A, Carr B I
Pittsburgh Transplantation Institute, University of Pittsburgh School of Medicine, PA, USA.
Biochem Biophys Res Commun. 1995 Nov 13;216(2):514-9. doi: 10.1006/bbrc.1995.2652.
Plasma levels of endothelin-1 (ET-1) are markedly higher in patients with hepatocellular carcinoma (HCC) than in normal controls. In order to further investigate this, we evaluated ET-1 immunoreactivity and mRNA expression in human HCC tissue. 70% (14/20) of the tumor tissues immunostained positively with ET-1 antibody and a significant association was observed between immunostaining in cells lining the tumor feeding vessels and tumor vascularity as determined by hepatic angiography. Moreover, the neoplastic hepatocytes in the tumors also stained positively with ET-1 antibody. All of the HCC tissue samples which immunostained for ET-1 also expressed ET-1 mRNA, indicating that ET-1 is actively synthesized by the tumors.
肝细胞癌（HCC）患者血浆中的内皮素 -1（ET-1）水平显著高于正常对照组。为进一步研究这一情况，我们评估了人HCC组织中ET-1的免疫反应性和mRNA表达。70%（14/20）的肿瘤组织经ET-1抗体免疫染色呈阳性，并且在肿瘤供血血管内衬细胞的免疫染色与通过肝血管造影确定的肿瘤血管形成之间观察到显著关联。此外，肿瘤中的肿瘤性肝细胞也经ET-1抗体染色呈阳性。所有经ET-1免疫染色的HCC组织样本也表达ET-1 mRNA，表明ET-1由肿瘤细胞主动合成。
