Nishi O, Nishi K, Fujiwara T, Shirasawa E
Nishi Eye Hospital, Osaka, Japan.
Br J Ophthalmol. 1995 Oct;79(10):939-43. doi: 10.1136/bjo.79.10.939.
AIMS/BACKGROUND: Residual lens epithelial cells (LECs) undergo fibrous proliferation after cataract surgery, resulting in capsular fibrosis. The purpose of this study was to determine the types of collagen produced in cultured LECs derived from human cataract LECs.
A circular section of the anterior capsule, about 5 mm in diameter, with LECs attached was obtained by anterior capsulotomy during cataract surgery and cultured directly without dispersion of the cells in a well, on the bottom of which a disc-shaped, thin plate of poly(methyl methacrylate) had been placed. At 5 to 6 weeks of culture, the proliferated cells of the culture were stained immunohistochemically with antibodies against human collagens I-VI by the avidin-biotin-peroxidase complex method.
Collagens I, IV, V, and VI were positive in the cultured cells. Types IV and V were strongly present in almost all the cells whereas types I and VI were only observed in a few cells. Collagens II and III were negative.
Since the lens capsule is known to be comprised of collagen IV, collagens I, V, and VI seem to be produced newly in culture. The capsular fibrosis seen after cataract surgery in vivo as a wound healing process of the lens capsule, may contain these types of collagens. The present culture model is useful for studying secondary cataract formation.
目的/背景：白内障手术后残留的晶状体上皮细胞（LECs）会发生纤维增生，导致囊膜纤维化。本研究的目的是确定源自人类白内障LECs的培养LECs中产生的胶原蛋白类型。
在白内障手术期间通过前囊切开术获取直径约5mm的附着有LECs的前囊圆形切片，并直接培养，细胞不分散于孔中，孔底部已放置盘状薄聚甲基丙烯酸甲酯板。培养5至6周时，通过抗生物素蛋白-生物素-过氧化物酶复合物法用抗人I-VI型胶原蛋白抗体对培养的增殖细胞进行免疫组织化学染色。
培养细胞中I、IV、V和VI型胶原蛋白呈阳性。IV型和V型在几乎所有细胞中强烈存在，而I型和VI型仅在少数细胞中观察到。II型和III型胶原蛋白呈阴性。
由于已知晶状体囊膜由IV型胶原蛋白组成，I、V和VI型胶原蛋白似乎是在培养中新产生的。白内障手术后在体内所见的囊膜纤维化作为晶状体囊膜的伤口愈合过程，可能包含这些类型的胶原蛋白。目前的培养模型对于研究继发性白内障的形成很有用。
