细胞黏菌苍白聚孢霉和盘基网柄菌中绿色荧光蛋白的产生
Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum.
作者信息
Fey P, Compton K, Cox E C
机构信息
Department of Molecular Biology, Princeton University, NJ 08544, USA.
出版信息
Gene. 1995 Nov 7;165(1):127-30. doi: 10.1016/0378-1119(95)00430-e.
PMID:7489901
Abstract
The green fluorescent protein-encoding gene from Aequorea victoria has been cloned into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorting (FACS).
摘要
来自维多利亚多管水母的绿色荧光蛋白编码基因已被克隆到几种不同的转化载体中，并在细胞黏菌苍白聚孢黏菌和盘基网柄菌中表达。我们发现该蛋白在这两个物种中都是稳定且无毒的，在活标本和固定标本中都能很容易地观察到，并且可用于通过荧光激活细胞分选（FACS）来纯化稀有细胞。
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