Coelho V, Moliterno R, Higuchi M L, Guilherme L, Cunha-Neto E, Fiorelli A I, Kalil J
Laboratory of Transplantation Immunology, University of São Paulo, Brazil.
Transplantation. 1995 Nov 15;60(9):980-4.
To investigate the role of gamma delta T cells in human heart transplantation, we searched for this T cell population in endomyocardial biopsies as well as in T cell lines and clones derived from graft-infiltrating lymphocytes. The number of gamma delta T cells in endomyocardial biopsies from transplanted patients (n = 55) was mostly low and did not differ significantly from nontransplanted patients (n = 21). Moreover, there was no association of gamma delta T cell distribution with rejection status or with time posttransplantation. Graft-derived T cell lines were established in the presence of autologous feeder cells and recombinant interleukin-2 to favor the growth of in vivo-activated T cells. Twenty T cell lines analyzed by flow cytometry showed low percentages of gamma delta T cells, and we were unable to obtain gamma delta T cell clones for functional studies. These results show that gamma delta T cells are poorly expressed on human heart allograft infiltrates and indicate that, when present, they are not activated in the graft. Our data suggest that gamma delta T cells do not have a major role in human heart rejection.
为了研究γδ T细胞在人类心脏移植中的作用，我们在内膜心肌活检组织以及从移植物浸润淋巴细胞衍生的T细胞系和克隆中寻找这种T细胞群体。移植患者（n = 55）内膜心肌活检组织中γδ T细胞的数量大多较低，与未移植患者（n = 21）相比无显著差异。此外，γδ T细胞分布与排斥反应状态或移植后时间均无关联。在自体饲养细胞和重组白细胞介素-2存在的情况下建立移植物来源的T细胞系，以促进体内活化T细胞的生长。通过流式细胞术分析的20个T细胞系显示γδ T细胞的百分比很低，并且我们无法获得用于功能研究的γδ T细胞克隆。这些结果表明，γδ T细胞在人类心脏同种异体移植物浸润中表达不佳，并且表明，当存在时，它们在移植物中未被激活。我们的数据表明，γδ T细胞在人类心脏排斥反应中没有主要作用。
