Vaessen L M, Ouwehand A J, Baan C C, Jutte N H, Balk A H, Claas F H, Weimar W
Department of Internal Medicine I, University Hospital Rotterdam-Dijkzigt, The Netherlands.
J Immunol. 1991 Aug 1;147(3):846-50.
Endomyocardial biopsies from human heart transplant recipients were investigated with respect to the occurrence of in vivo activated, alloreactive TCR-gamma delta+ cells. More than one year after transplantation, 30% of the biopsy-derived T cell cultures contained TCR-gamma delta+ cells, whereas in the first year after heart transplant in only 8% of the culture TCR-gamma delta+ cells were found. Such an increase of TCR-gamma delta+ cells was not observed in the peripheral blood of the patient. In most biopsy-derived cultures, the gamma delta cells were delta-TCS1+. No donor-specific cytotoxic activity could be demonstrated for TCR-gamma delta+ cells tested, whereas non-MHC-restricted cytotoxicity was found in several cultures. The occurrence of nonalloreactive TCR-gamma delta+ cells late after transplantation, when acute cellular rejection episodes are rare, suggests a role in the down-regulation of the allo-Ir.
对人类心脏移植受者的心肌内膜活检组织进行研究，以了解体内活化的同种异体反应性TCR-γδ+细胞的发生情况。移植一年多后，30%的活检来源的T细胞培养物中含有TCR-γδ+细胞，而在心脏移植后的第一年，只有8%的培养物中发现了TCR-γδ+细胞。在患者外周血中未观察到这种TCR-γδ+细胞的增加。在大多数活检来源的培养物中，γδ细胞为δ-TCS1+。对于所检测的TCR-γδ+细胞，未显示出供体特异性细胞毒性活性，而在几种培养物中发现了非MHC限制的细胞毒性。在移植后期急性细胞排斥反应罕见时出现非同种异体反应性TCR-γδ+细胞，提示其在同种异体免疫反应下调中起作用。
