一个携带金属β-内酰胺酶基因blaIMP的新型整合子样元件。
A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP.
作者信息
Arakawa Y, Murakami M, Suzuki K, Ito H, Wacharotayankun R, Ohsuka S, Kato N, Ohta M
机构信息
Department of Bacteriology, Nagoya University School of Medicine, Japan.
出版信息
Antimicrob Agents Chemother. 1995 Jul;39(7):1612-5. doi: 10.1128/AAC.39.7.1612.
Abstract
A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was located in the space between an integrase-like gene and an aac(6')-Ib-like gene. The deduced amino acid sequence for the putative integrase gene showed considerable identity (60.9%) to that of the Escherichia coli integrase reported. Sequences similar to the GTTRRRY and an atypical 59-base element containing a 67-bp inverted repeat sequence, which were peculiar to the integrase-dependent recombination, were also conserved in the flanking regions of the blaIMP gene. These findings imply that the metallo-beta-lactamase gene in S. marcescens AK9373 is carried by a novel integron-like element that is mediated by a transferable large plasmid.
摘要
从一株耐碳青霉烯类的粘质沙雷氏菌AK9373中克隆出一个质粒介导的金属β-内酰胺酶基因。该金属β-内酰胺酶基因与blaIMP相同，位于一个整合酶样基因和一个aac(6')-Ib样基因之间。推测的整合酶基因推导氨基酸序列与已报道的大肠杆菌整合酶有相当高的同源性（60.9%）。与整合酶依赖性重组特有的GTTRRRY以及含有67bp反向重复序列的非典型59碱基元件相似的序列，在blaIMP基因的侧翼区域也保守。这些发现表明，粘质沙雷氏菌AK9373中的金属β-内酰胺酶基因由一个可转移的大质粒介导的新型整合子样元件携带。
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