Tachibana Y, Kawanishi K
Kobe Women's College of Pharmacy, Japan.
Planta Med. 1992 Jun;58(3):250-4. doi: 10.1055/s-2006-961446.
Eighteen different crude drugs were extracted with hot water and saline, and protein fractions were prepared from the extracts by ammonium sulfate precipitation. Mitogenic activities of the protein fractions were examined both on human peripheral blood lymphocytes and on mouse spleen cells. Potent mitogenic activities for both human and/or mouse lymphocytes were found in the protein fractions of four crude drugs, namely, Bupleuri radix, Pinelliae tuber, Sophorae radix, and Zedoariae rhizoma. Target specificities of these mitogens were investigated by using isolated T and B cells and lymphocytes from athymic nude mice. Sensitivity to protease digestion as well as water-soluble, ammonium sulfate precipitable nature assures that the substances responsible for the mitogenic activities are proteins.
用热水和盐水对18种不同的天然药物进行提取，并通过硫酸铵沉淀从提取物中制备蛋白质组分。检测了这些蛋白质组分对人外周血淋巴细胞和小鼠脾细胞的促有丝分裂活性。在四种天然药物（即柴胡、半夏、苦参和莪术）的蛋白质组分中发现了对人和/或小鼠淋巴细胞具有强效的促有丝分裂活性。通过使用分离的T细胞、B细胞以及无胸腺裸鼠的淋巴细胞，研究了这些有丝分裂原的靶细胞特异性。对蛋白酶消化的敏感性以及水溶性、硫酸铵可沉淀的性质确保了负责促有丝分裂活性的物质是蛋白质。
