Hannant D, Else R W, Crighton G W
Vet Rec. 1978 Nov 11;103(20):441-3. doi: 10.1136/vr.103.20.441.
Soluble proteins were prepared from canine spontaneous mammary carcinoma (CSMC) cells by extraction with 3M KCl or limited hydrolysis of isolated cell membranes with papain. These preparations were assayed for tumour-associated antigens by inhibition of fully absorbed rabbit antisera. Organ-specific reactivity was demonstrated and antigenic specificity indicated the possible involvement of fetal components. Exclusion chromatography showed that antigenic activity was associated with macromolecules of different sizes in each of the protein preparations which allowed partial purification of tumour-associated antigens. Antigens were also prepared from circulating immune complexes in the serum of tumour-bearing animals. These studies provide a basis for the development of serological markers of malignancy in CSMC.
通过用3M氯化钾提取或用木瓜蛋白酶对分离的细胞膜进行有限水解，从犬自发性乳腺癌（CSMC）细胞中制备可溶性蛋白质。通过抑制完全吸收的兔抗血清对这些制剂进行肿瘤相关抗原检测。证实了器官特异性反应性，抗原特异性表明可能涉及胎儿成分。排阻色谱显示，每种蛋白质制剂中的抗原活性与不同大小的大分子相关，这使得肿瘤相关抗原得以部分纯化。还从荷瘤动物血清中的循环免疫复合物中制备了抗原。这些研究为开发CSMC恶性肿瘤的血清学标志物提供了基础。
