Neri D, Petrul H, Roncucci G
Cambridge Centre for Protein Engineering, MRC Centre, United Kingdom.
Cell Biophys. 1995 Aug;27(1):47-61. doi: 10.1007/BF02822526.
Recombinant antibody fragments binding with high affinity to their target can be obtained either from hybridomas or directly from antibody libraries on filamentous phage. These fragments are devoid of any activity other than antigen binding, and have to be processed and functionalized in order to be suitable for clinical applications. This article presents the authors' view on the procedures and the features that are important for effective transformation of recombinant antibodies into useful immunotherapeutic agents. The topics presented include phage display methodologies, engineering of high-affinity binding, purification, and functionalization strategies of recombinant antibodies.
与靶标具有高亲和力结合的重组抗体片段可以从杂交瘤中获得，也可以直接从丝状噬菌体上的抗体文库中获得。这些片段除了抗原结合外没有任何其他活性，必须进行加工和功能化才能适用于临床应用。本文介绍了作者对于将重组抗体有效转化为有用的免疫治疗剂的程序和重要特征的观点。所呈现的主题包括噬菌体展示方法、高亲和力结合的工程改造、重组抗体的纯化和功能化策略。
