Liberatore M, Neri D, Neri G, Pini A, Iurilli A P, Ponzo F, Spampinato G, Padula F, Pala A, Colella A C
Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita' di Roma "La Sapienza", Via Regina Elena 324, I-00161, Roma, Italy.
Eur J Nucl Med. 1995 Nov;22(11):1326-9. doi: 10.1007/BF00801622.
High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability.
如今，高亲和力的细菌表达抗体片段可以从已有的杂交瘤中克隆出来，或者更方便地直接从丝状噬菌体展示的抗体库中分离得到。这类抗体可以用含有一个半胱氨酸残基的C末端肽标签进行标记，这为包括锝-99m标记在内的多种应用提供了一个便利的功能化位点。在此，我们描述了一种简单的一步法，用于用半胱氨酸标记的重组抗体进行锝-99m标记，放射性核素掺入率超过50%。标记后的抗体完全保留了免疫反应性且稳定性良好。
