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从临床正常、自由放养的津巴布韦野生有蹄类动物的血液和骨髓样本中检测反刍兽考德里氏体。

Detection of Cowdria ruminantium in blood and bone marrow samples from clinically normal, free-ranging Zimbabwean wild ungulates.

作者信息

Kock N D, van Vliet A H, Charlton K, Jongejan F

机构信息

Department of Paraclinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, Harare, Zimbabwe.

出版信息

J Clin Microbiol. 1995 Sep;33(9):2501-4. doi: 10.1128/jcm.33.9.2501-2504.1995.

DOI:10.1128/jcm.33.9.2501-2504.1995
PMID:7494060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228457/
Abstract

Cowdria ruminantium causes severe, often fatal disease in domestic ruminants, whereas wildlife species usually are not affected. Blood and bone marrow samples from healthy, free-ranging Zimbabwean ungulates were taken during translocation from areas harboring Amblyomma ticks and tested for the presence of C. ruminantium, using a PCR assay based on the C. ruminantium map1 gene. Positive reactions were obtained in tsessebe (Damaliscus lunatus), waterbuck (Kobus ellipsiprymnus), and impala (Aepyceros melampus). Wildlife species may therefore be a reservoir for C. ruminantium thus contributing to the spread of cowdriosis.

摘要

反刍动物考德里氏体可导致家养反刍动物患上严重且往往致命的疾病，而野生动物通常不受影响。在将健康的、自由放养的津巴布韦有蹄类动物从携带钝缘蜱的地区进行转移期间，采集了血液和骨髓样本，并使用基于反刍动物考德里氏体map1基因的聚合酶链反应（PCR）检测法检测是否存在反刍动物考德里氏体。在转角牛羚（白纹牛羚）、水羚（水羚属）和黑斑羚（黑斑羚属）中获得了阳性反应。因此，野生动物物种可能是反刍动物考德里氏体的宿主，从而导致心水病的传播。

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本文引用的文献

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An immunoblotting diagnostic assay for heartwater based on the immunodominant 32-kilodalton protein of Cowdria ruminantium detects false positives in field sera.一种基于反刍动物考德里氏体免疫显性32千道尔顿蛋白的牛心水病免疫印迹诊断检测方法在现场血清中检测到假阳性。
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The immunodominant 32-kilodalton protein of Cowdria ruminantium is conserved within the genus Ehrlichia.反刍动物考德里氏体的免疫显性32千道尔顿蛋白在埃立克体属内保守。
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Molecular cloning, sequence analysis, and expression of the gene encoding the immunodominant 32-kilodalton protein of Cowdria ruminantium.反刍动物考德里氏体免疫显性32千道尔顿蛋白编码基因的分子克隆、序列分析及表达
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Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis.白尾鹿（弗吉尼亚白尾鹿）对人埃立克体病的病原体查菲埃立克体感染的易感性。
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