抗猪痢疾蛇形螺旋体和无害蛇形螺旋体单克隆抗体的制备、特性鉴定及其在血清分型中的应用。
Production and characterization of monoclonal antibodies against Serpulina hyodysenteriae and S. innocens and their use in serotyping.
作者信息
Achacha M, Mittal K R
机构信息
Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2519-21. doi: 10.1128/jcm.33.9.2519-2521.1995.
Murine monoclonal antibodies (MAbs) directed against serotypes 1, 2, 8, and 9 of Serpulina hyodysenteriae and strain B256 of Serpulina innocens were produced and characterized. A serological classification of 96 field strains of S. hyodysenteriae and 28 field strains of S. innocens isolated from pigs showing clinical signs of swine dysentery was performed by rapid dot enzyme-linked immunosorbent assay (ELISA) with the MAbs. The results indicated that the majority of the field strains of S. hyodysenteriae (69%) belonged to serotypes 8, 1, and 9, whereas only 31% of the S. innocens strains were recognized by MAb 9H7, indicating the presence of antigenic heterogeneity among S. innocens isolates. Rapid dot ELISA with type-specific MAbs was found to be specific, sensitive, and easy to perform and thus to be suitable for routine serotyping of S. hyodysenteriae and S. innocens isolates. This is the first report of MAbs being used for serotyping clinical isolates of S. hyodysenteriae and S. innocens.
制备并鉴定了针对猪痢疾蛇形螺旋体血清型1、2、8和9以及无害蛇形螺旋体菌株B256的鼠单克隆抗体（MAb）。采用这些单克隆抗体，通过快速斑点酶联免疫吸附测定（ELISA）对从表现出猪痢疾临床症状的猪中分离出的96株猪痢疾蛇形螺旋体和28株无害蛇形螺旋体田间菌株进行了血清学分类。结果表明，大多数猪痢疾蛇形螺旋体田间菌株（69%）属于血清型8、1和9，而只有31%的无害蛇形螺旋体菌株能被单克隆抗体9H7识别，这表明无害蛇形螺旋体分离株之间存在抗原异质性。发现用型特异性单克隆抗体进行快速斑点ELISA具有特异性、敏感性且易于操作，因此适用于猪痢疾蛇形螺旋体和无害蛇形螺旋体分离株的常规血清分型。这是关于单克隆抗体用于猪痢疾蛇形螺旋体和无害蛇形螺旋体临床分离株血清分型的首次报道。
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