用于检测猪痢疾密螺旋体抗原抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection of antibody to Treponema hyodysenteriae antigens.
作者信息
Joens L A, Nord N A, Kinyon J M, Egan I T
出版信息
J Clin Microbiol. 1982 Feb;15(2):249-52. doi: 10.1128/jcm.15.2.249-252.1982.
The enzyme-linked immunosorbent assay (ELISA) was evaluated and compared with the microtitration agglutination test for the detection of swine antibody to Treponema hyodysenteriae lipopolysaccharide antigens. Cells of T. hyodysenteriae serotypes 1 and 2 were extracted with hot phenol-water (68 degrees C). The lipopolysaccharide fraction from the aqueous phase was coated on plastic wells at concentrations of 1 micrograms (serotype 1) and 10 micrograms (serotype 2) of carbohydrate per ml. The ELISA was serotype specific when lipopolysaccharide antigens were reacted against sera from convalescent swine. Seroconversion of infected pigs was detectable with the ELISA within 1 to 2 weeks postinoculation and with the microtitration agglutination test 2 to 3 weeks postinoculation. Antibody titers could be detected in convalescent pigs as long as 19 weeks postinoculation by the ELISA and 12 to 13 weeks postinoculation by the microtitration agglutination test. Therefore, the ELISA may be useful for the detection of asymptomatic carriers.
对酶联免疫吸附测定（ELISA）进行了评估，并与微量滴定凝集试验进行比较，以检测猪针对猪痢疾密螺旋体脂多糖抗原的抗体。用热酚水（68摄氏度）提取猪痢疾密螺旋体1型和2型的细胞。将水相中的脂多糖部分以每毫升1微克（1型血清型）和10微克（2型血清型）碳水化合物的浓度包被在塑料孔中。当脂多糖抗原与康复猪的血清反应时，ELISA具有血清型特异性。用ELISA在接种后1至2周可检测到感染猪的血清转化，用微量滴定凝集试验在接种后2至3周可检测到。通过ELISA在接种后长达19周、通过微量滴定凝集试验在接种后12至13周可在康复猪中检测到抗体滴度。因此，ELISA可能有助于检测无症状携带者。
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