利用脂多糖特异性单克隆抗体鉴定一组新型猪痢疾蛇形螺旋体分离株。
Identification of a novel group of Serpulina hyodysenteriae isolates by using a lipopolysaccharide-specific monoclonal antibody.
作者信息
Alderton M R, Smith S C, Coloe P J
机构信息
Department of Applied Biology, Royal Melbourne Institute of Technology, Victoria, Australia.
出版信息
J Clin Microbiol. 1993 May;31(5):1326-8. doi: 10.1128/jcm.31.5.1326-1328.1993.
Abstract
A monoclonal antibody to Serpulina hyodysenteriae 8930 was produced and was used to probe pronase-treated cell lysates of S. hyodysenteriae isolates in immunblots. The results showed that the monoclonal antibody was specific for only five closely related S. hyodysenteriae isolates: 8930, 5380, 70A, RMIT 88, and RMIT 97.
摘要
制备了一种针对猪痢疾蛇形螺旋体8930的单克隆抗体，并用于在免疫印迹中探测经链霉蛋白酶处理的猪痢疾蛇形螺旋体分离株的细胞裂解物。结果表明，该单克隆抗体仅对5株密切相关的猪痢疾蛇形螺旋体分离株具有特异性：8930、5380、70A、RMIT 88和RMIT 97。
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