Kigure T, Saito A, Seida K, Yamada S, Ishihara K, Okuda K
Department of Periodontics, Tokyo Dental College, Chiba, Japan.
J Periodontal Res. 1995 Sep;30(5):332-41. doi: 10.1111/j.1600-0765.1995.tb01284.x.
Localization of Porphyromonas gingivalis and Treponema denticola in different areas of subgingival plaque from advanced adult periodontitis patients was studied immunohistochemically using sensitive immunogold-silver staining and immunoelectron microscopy. Fourteen periodontally diseased teeth were extracted without damaging the subgingival plaque, fixed, and embedded. The subgingival plaque samples were sectioned according to four different pocket depths (0-2 mm, 2-4 mm, 4-6 mm and > or = 6 mm). Serial thin sections were stained using specific antisera to P. gingivalis or T. denticola and then with secondary antibody labelled with colloidal-gold. Cells of both P. gingivalis and T. denticola were predominantly found in subgingival plaque located at depths of more than 4 mm in periodontal pockets. T. denticola cells were found in the surface layers of subgingival plaque, and P. gingivalis were predominant beneath them. However, in the deeper subgingival plaque, the coexistence of P. gingivalis and T. denticola was observed. The present findings suggest that P. gingivalis and T. denticola play important roles in the pathogenicity of periodontal disease and provide the useful information for elucidating the pattern of colonization of microorganisms in the periodontal pocket.
采用灵敏的免疫金银染色和免疫电子显微镜技术，对晚期成人牙周炎患者龈下菌斑不同区域牙龈卟啉单胞菌和具核梭杆菌进行免疫组织化学定位研究。14颗患牙周病的牙齿在不损伤龈下菌斑的情况下拔除，固定并包埋。龈下菌斑样本根据4种不同的牙周袋深度（0 - 2mm、2 - 4mm、4 - 6mm和≥6mm）进行切片。连续超薄切片用牙龈卟啉单胞菌或具核梭杆菌的特异性抗血清染色，然后用胶体金标记的二抗染色。牙龈卟啉单胞菌和具核梭杆菌细胞主要存在于牙周袋深度超过4mm的龈下菌斑中。具核梭杆菌细胞见于龈下菌斑表层，其下方以牙龈卟啉单胞菌为主。然而，在更深层的龈下菌斑中，观察到牙龈卟啉单胞菌和具核梭杆菌共存。本研究结果表明，牙龈卟啉单胞菌和具核梭杆菌在牙周病致病性中起重要作用，并为阐明牙周袋中微生物的定植模式提供了有用信息。
