反转录转座子Ty1中的正链强终止DNA合成。
Plus-strand strong-stop DNA synthesis in retrotransposon Ty1.
作者信息
Lauermann V, Nam K, Trambley J, Boeke J D
机构信息
Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
出版信息
J Virol. 1995 Dec;69(12):7845-50. doi: 10.1128/JVI.69.12.7845-7850.1995.
Abstract
Reverse transcription in the yeast retrotransposon Ty1 follows the general "rules" of retroviral replication overall. However, some details of the retroviral and Ty1 reverse transcription processes are different. We have identified and determined the structure of plus-strand strong-stop DNA and examined the effect of polypurine tract deletion mutations on its synthesis. Furthermore, we have defined the stop signal for plus-strand strong-stop DNA synthesis as an unusual 2'-O-ribosylated nucleotide in the primer tRNA. Full-length plus-strand strong-stop DNA, following strand transfer, would have a terminal 2-base mismatch with minus-strand DNA. These findings indicate that the mechanism of plus-strand strong-stop DNA transfer in Ty1 differs from that of the retroviral transfer and suggest that full-length plus-strand strong-stop DNA is not a direct intermediate in Ty1 retrotransposition.
摘要
酵母逆转座子Ty1中的逆转录总体上遵循逆转录病毒复制的一般“规则”。然而，逆转录病毒和Ty1逆转录过程的一些细节有所不同。我们已经鉴定并确定了正链强终止DNA的结构，并研究了聚嘌呤序列缺失突变对其合成的影响。此外，我们将正链强终止DNA合成的终止信号定义为引物tRNA中一种不寻常的2'-O-核糖基化核苷酸。链转移后，全长正链强终止DNA与负链DNA会有一个末端2碱基错配。这些发现表明，Ty1中正链强终止DNA转移的机制与逆转录病毒转移的机制不同，并表明全长正链强终止DNA不是Ty1逆转座过程中的直接中间体。
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本文引用的文献
1
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Nucleic Acids Res. 1993 Jul 25;21(15):3513-20. doi: 10.1093/nar/21.15.3513.
2
The yeast initiator tRNAMet can act as an elongator tRNA(Met) in vivo.酵母起始tRNAMet在体内可作为延伸tRNA(Met)发挥作用。
J Mol Biol. 1993 Sep 5;233(1):43-58. doi: 10.1006/jmbi.1993.1483.
3
In-frame linker insertion mutagenesis of yeast transposon Ty1: phenotypic analysis.酵母转座子Ty1的框内接头插入诱变：表型分析
Gene. 1994 Feb 11;139(1):19-26. doi: 10.1016/0378-1119(94)90518-5.
4
Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination.Rit1是一种可介导起始tRNA和延伸tRNA识别的tRNA骨架修饰酶。
Cell. 1994 Nov 4;79(3):535-46. doi: 10.1016/0092-8674(94)90262-3.
5
The primer tRNA sequence is not inherited during Ty1 retrotransposition.在Ty1逆转录转座过程中，引物tRNA序列不会遗传。
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9847-51. doi: 10.1073/pnas.91.21.9847.
6
Yeast lariat debranching enzyme. Substrate and sequence specificity.酵母套索脱支酶。底物与序列特异性。
J Biol Chem. 1994 Aug 12;269(32):20613-21.
7
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J Biol Chem. 1994 Feb 11;269(6):3925-7.
8
Synthesis and integration of avian sarcoma virus DNA.禽肉瘤病毒DNA的合成与整合
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:1091-6. doi: 10.1101/sqb.1980.044.01.117.
9
The SPT3 gene is required for normal transcription of Ty elements in S. cerevisiae.SPT3基因是酿酒酵母中Ty元件正常转录所必需的。
Cell. 1984 Dec;39(3 Pt 2):675-82. doi: 10.1016/0092-8674(84)90474-4.
10
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型筛选的快速高效位点特异性诱变。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
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