Taylor J M, Hsu T W, Yeater C, Mason W S
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:1091-6. doi: 10.1101/sqb.1980.044.01.117.
In this paper, we have presented data regarding the integration of viral DNA and the persistence of unintegrated viral DNA. We know that integration occurs with specificity with respect to the viral genome, and yet it remains to be established which, if any, of the unintegrated DS DNA species-either the linear or the covalently closed circular forms-is the immediate precursor to the DNA that does become integrated. The results of the present studies indicate that the viral DNA species that are able to persist in an unintegrated state may be transcribed to yield new virions. Furthermore, these species may not be intermediates as far as the integration pathway, but may instead be terminal products of a synthesis reaction that has proceeded beyond the point where integration normally occurs. Varmus et al. (1977) have shown that integration occurs only into newly replicated cellular DNA. Therefore, we would speculate that integration may occur by an interaction between a replication fork of the host cell DNA and an earlier, non-double-strand form of viral DNA.
在本文中，我们展示了有关病毒DNA整合以及未整合病毒DNA持续性的数据。我们知道，整合是相对于病毒基因组特异性发生的，但仍有待确定未整合的双链DNA物种（无论是线性形式还是共价闭合环状形式）中，哪一种（如果有的话）是最终整合的DNA的直接前体。本研究结果表明，能够以未整合状态持续存在的病毒DNA物种可能会被转录产生新的病毒粒子。此外，就整合途径而言，这些物种可能不是中间体，而是合成反应的终产物，该合成反应已经进行到超出正常发生整合的阶段。瓦尔穆斯等人（1977年）已经表明，整合仅发生在新复制的细胞DNA中。因此，我们推测整合可能是通过宿主细胞DNA的复制叉与病毒DNA的早期非双链形式之间的相互作用而发生的。
