Jaber M, Normand E, Bloch B
E.P. 74 CNRS, Laboratoire d'Histologie-Embryologie (U.F.R. II), Université de Bordeaux II, France.
Brain Res Mol Brain Res. 1995 Aug;32(1):156-60. doi: 10.1016/0169-328x(95)00064-y.
We investigated the molecular mechanisms responsible for the preproenkephalin A mRNA increase following catecholamine depletion by reserpine using quantitative in situ hybridization at the cellular level. Macroscopic analysis showed that short term reserpine treatment increases the preproenkephalin A mRNA level in the rat striatum to +40.2 +/- 9%. Microautoradiography analysis demonstrated different increases in the preproenkephalin A mRNA level in different parts of the striatum: +124 +/- 22% in the dorso-median striatum, +131 +/- 19% in the dorso-lateral striatum, +119 +/- 8% in the ventro-lateral striatum and +75 +/- 6% in the ventro-median striatum. We found no difference in the number of cells expressing PPA mRNA in reserpine treated rats suggesting that these increases are only due to an increase in the number of mRNA expressed by cell.
我们使用细胞水平的定量原位杂交技术，研究了利血平导致儿茶酚胺耗竭后前脑啡肽原A mRNA增加的分子机制。宏观分析表明，短期利血平处理可使大鼠纹状体中的前脑啡肽原A mRNA水平提高至+40.2±9%。微放射自显影分析显示，纹状体不同部位的前脑啡肽原A mRNA水平有不同程度的升高：背内侧纹状体升高+124±22%，背外侧纹状体升高+131±19%，腹外侧纹状体升高+119±8%，腹内侧纹状体升高+75±6%。我们发现利血平处理的大鼠中表达PPA mRNA的细胞数量没有差异，这表明这些增加仅仅是由于细胞表达的mRNA数量增加所致。
