Pelouch V, Milerová M, Ostádal B, Hucín B, Samánek M
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Mol Cell Biochem. 1995;147(1-2):43-9. doi: 10.1007/BF00944782.
The purpose of the present study was to compare protein profiling of atria and ventricles in children operated for congenital heart disease. Tissue samples were obtained during surgery from patients with normoxemic (ventricular and atrial septal defects) and hypoxemic (tetralogy of Fallot) diseases. Protein fractions were isolated by stepwise extraction from both right ventricular and atrial musculature. The concentration of total atrial protein in the normoxemic patients exceeded the ventricular value (110 +/- 2.1 vs 99.9 +/- 4.0 mg.g-1 wet weight, respectively); in the hypoxemic group this atrio-ventricular difference disappeared. The concentration of contractile proteins in all cardiac samples was significantly higher in the ventricles as compared with atria, while the concentration of collagenous proteins was significantly higher in the atria (due to a higher amount of the insoluble collagenous fraction). The concentration of sarcoplasmic proteins (containing predominantly enzyme systems for aerobic and anaerobic substrate utilization), however did not differ between ventricles and atria. Furthermore, ventricular contractile fractions obtained from both normoxemic and hypoxemic patients were contaminated with the myosin light chain of atrial origin. Soluble collagenous fractions (containing newly synthesized collagenous proteins, predominantly collagen I and III), derived from all ventricular samples, were contaminated by low molecular weight fragments (mol. weight 29-35 kDa). The proportion of the soluble collagenous fraction was significantly higher in atrial but not in ventricular myocardium of hypoxemic children as compared with the normoxemic group. It seems, therefore, that lower oxygen saturation affects the synthesis of collagen preferentially in atrial tissue.
本研究的目的是比较接受先天性心脏病手术的儿童心房和心室的蛋白质谱。在手术过程中从患有正常血氧（室间隔和房间隔缺损）和低氧血症（法洛四联症）疾病的患者身上获取组织样本。通过从右心室和心房肌肉组织逐步提取来分离蛋白质组分。正常血氧患者的心房总蛋白浓度超过心室值（分别为110±2.1与99.9±4.0mg·g-1湿重）；在低氧血症组中，这种房室差异消失。与心房相比，所有心脏样本中的收缩蛋白浓度在心室中显著更高，而胶原蛋白浓度在心房中显著更高（由于不溶性胶原部分含量更高）。然而，肌浆蛋白（主要包含有氧和无氧底物利用的酶系统）的浓度在心室和心房之间没有差异。此外，从正常血氧和低氧血症患者获得的心室收缩组分都被心房来源的肌球蛋白轻链污染。来自所有心室样本的可溶性胶原组分（包含新合成的胶原蛋白，主要是I型和III型胶原）被低分子量片段（分子量29 - 35kDa）污染。与正常血氧组相比，低氧血症儿童心房心肌中的可溶性胶原组分比例显著更高，但在心室心肌中并非如此。因此，似乎较低的氧饱和度优先影响心房组织中胶原的合成。
