Bossù P, Visconti U, Ruggiero P, Macchia G, Muda M, Bertini R, Bizzarri C, Colagrande A, Sabbatini V, Maurizi G
Research Center Dompé SpA, L'Aquila, Italy.
Am J Pathol. 1995 Dec;147(6):1852-61.
Of the two known types of specific receptors for interleukin (IL)-1, the function of the type II IL-1 receptor (IL-1RII) is still elusive. IL-1RII is allegedly devoid of signaling capacity and is therefore thought to act by trapping and inhibiting IL-1. To directly assess the functional role of IL-1RII, a human keratinocyte cell line has been stably transfected with a cDNA coding for IL-1RII, and its responsiveness to IL-1 has been compared with that of nontransfected cells. Parental cells express IL-1RI and are responsive to low doses of IL-1, whereas transfected cells overexpress IL-1RII, both in its membrane and soluble form, and show a dramatically impaired response to IL-1. Selective block of IL-1RII restores the ability of transfected keratinocytes to respond to IL-1, indicating that the overexpressed IL-1RII is in fact uniquely responsible for their refractoriness to IL-1. The main mechanism of unresponsiveness in transfected keratinocytes appears to be the capture and neutralization of IL-1 by the soluble form of IL-1RII.
在白细胞介素（IL）-1的两种已知特异性受体类型中，II型IL-1受体（IL-1RII）的功能仍不清楚。据称IL-1RII缺乏信号传导能力，因此被认为通过捕获和抑制IL-1发挥作用。为了直接评估IL-1RII的功能作用，用人角质形成细胞系稳定转染编码IL-1RII的cDNA，并将其对IL-1的反应性与未转染细胞进行比较。亲代细胞表达IL-1RI，对低剂量IL-1有反应，而转染细胞在膜和可溶性形式上均过度表达IL-1RII，并对IL-1的反应显著受损。选择性阻断IL-1RII可恢复转染角质形成细胞对IL-1的反应能力，表明过度表达的IL-1RII实际上是其对IL-1难治性的唯一原因。转染角质形成细胞无反应的主要机制似乎是可溶性形式的IL-1RII捕获和中和IL-1。
