在耐多药结核病高发地区，将INNO-LiPA Rif.TB线性探针检测法直接应用于360份涂片阳性呼吸道标本，以快速鉴定结核分枝杆菌复合群菌株并检测利福平耐药性。
Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis.
作者信息
Viveiros Miguel, Leandro Clara, Rodrigues Liliana, Almeida Josefina, Bettencourt Rosário, Couto Isabel, Carrilho Lurdes, Diogo José, Fonseca Ana, Lito Luís, Lopes João, Pacheco Teresa, Pessanha Mariana, Quirim Judite, Sancho Luísa, Salfinger Max, Amaral Leonard
机构信息
Unidade de Micobacterias do Instituto de Higiene e Medicina Tropical, Lisboa, Portugal.
出版信息
J Clin Microbiol. 2005 Sep;43(9):4880-4. doi: 10.1128/JCM.43.9.4880-4884.2005.
Abstract
The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.
摘要
采用INNO-LiPA Rif.TB检测法对结核分枝杆菌复合群菌株进行鉴定并检测利福平（RIF）耐药性，该检测法已在耐多药结核病（MDR-TB）高发地区的360份涂片阳性呼吸道标本中进行了评估。与传统鉴定/培养方法相比，其灵敏度为82.2%，特异性为66.7%；检测RIF耐药性的灵敏度和特异性分别为100.0%和96.9%。该检测法有可能提供快速信息，这对MDR-TB的有效管理至关重要。
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