Gilleron M, Puzo G
Laboratoire de Pharmacologie et de Toxicologie Fondamentales du Centre National de la Recherche Scientifique, Toulouse, France.
Glycoconj J. 1995 Jun;12(3):298-308. doi: 10.1007/BF00731333.
A set of Lipooligosaccharides (LOSs) has previously been characterized in M. gastri W471. The structure of the highly antigenic LOS (LOS-III) was elucidated and this molecule can unambiguously distinguish M. gastri from the opportunistic pathogen M. kansasii. In the present study, the structures of three other M. gastri W471 LOSs were determined by one-dimensional 1H-NMR spectroscopy and gas liquid chromatography. They differ by the number of Xylp units and by the structure of the distal monosaccharide. The two dimensional (2D) NMR approach was successfully applied to the LOS antigen of M. kansasii to locate the acetyl and acyl substituents and to determine the anomeric configuration of the alpha-D-Fucp unit. The molecular specificity of anti-LOS-III antibodies was investigated and the LOS-III epitope was defined as the distal disaccharide: 3,6-dideoxy-4-C-(1,3-dimethoxy-4,5,6,7-tetrahydroxy- heptyl)-alpha-xylohexp-(1-->3)-beta-L-Xylp.
先前已对胃分支杆菌W471中的一组脂寡糖（LOSs）进行了表征。已阐明高抗原性LOS（LOS-III）的结构，该分子可明确区分胃分支杆菌与机会致病菌堪萨斯分支杆菌。在本研究中，通过一维1H-NMR光谱和气相色谱法确定了胃分支杆菌W471的其他三种LOS的结构。它们在木糖（Xylp）单元的数量和末端单糖的结构上有所不同。二维（2D）NMR方法成功应用于堪萨斯分支杆菌的LOS抗原，以定位乙酰基和酰基取代基，并确定α-D-岩藻糖（α-D-Fucp）单元的异头构型。研究了抗LOS-III抗体的分子特异性，并将LOS-III表位定义为末端二糖：3,6-二脱氧-4-C-（1,3-二甲氧基-4,5,6,7-四羟基庚基）-α-木糖己糖-（1→3）-β-L-木糖。
