Bentivoglio M, Grassi-Zucconi G, Peng Z C, Kristensson K
Institute of Anatomy and Histology, Medical faculty, University of Verona, Italy.
Bull Soc Pathol Exot. 1994;87(5):372-5.
The rest-activity and body temperature 24 hours cycles, as well as the pattern of spontaneous sleep, were investigated in rats after infection with Trypanosoma brucei brucei. In the infected rats, which were entrained to a 12 hours/12 hours photoperiod, a considerable hypokinesia was detected during the hours of darkness. In most of the infected animals, the body temperature cycle displayed a lower amplitude and an advance of about 3 hours in respect to control rats; in addition, the body temperature rhythm was not significant in some infected rats. The relative proportion of slow wave synchronized sleep, as well as the rapid-eye movement (REM) latency, were significantly reduced in the infected animals, in which sleep was considerably fragmented. The induction of Fos (the protein encoded by the immediate early gene c-fos), in response to light stimulation during the early subjective night, was severely impaired in the hypothalamic suprachiasmatic nuclei in trypanosome-infected rats. Altogether these data point out a disruption of locomotor activity and body temperature 24 hours cycle and a major disorganization of sleep during experimental trypanosomiasis. In addition, our findings indicate that the molecular and functional correlates of the synchronizing action of the suprachiasmatic nuclei, which play a major role of biological clock of endogenous biological rhythms, could be altered during trypanosome infection.
在感染布氏布氏锥虫的大鼠中，研究了其静息-活动和24小时体温周期，以及自发睡眠模式。在被诱导进入12小时/12小时光周期的感染大鼠中，在黑暗时段检测到明显的运动减退。在大多数感染动物中，体温周期相对于对照大鼠显示出较低的振幅和大约提前3小时；此外，一些感染大鼠的体温节律不明显。感染动物中慢波同步睡眠的相对比例以及快速眼动（REM）潜伏期显著降低，其中睡眠严重碎片化。在锥虫感染的大鼠中，在下丘脑视交叉上核中，对主观夜间早期光刺激的Fos（立即早期基因c-fos编码的蛋白质）诱导严重受损。总之，这些数据表明在实验性锥虫病期间，运动活动和24小时体温周期受到破坏，睡眠出现严重紊乱。此外，我们的研究结果表明，视交叉上核同步作用的分子和功能相关性（视交叉上核对内源性生物节律的生物钟起主要作用）在锥虫感染期间可能会改变。
