Candiloros H, Muller S, Zeghari N, Donner M, Drouin P, Ziegler O
Départment de Nutrition, Centre Hospitalo-Universitaire, Nancy, France.
Diabetes Care. 1995 Apr;18(4):549-51. doi: 10.2337/diacare.18.4.549.
To examine the factors that might alter the fluidity of erythrocyte membrane in insulin-dependent diabetes mellitus (IDDM) patients.
The subjects were 10 health men and 30 IDDM mem: 10 with good blood glucose (BG) control (HbA1c 5.88 +/- 0.60% [mean +/- SD]), 10 with poor BG control (HbA1C 9.48 +/- 1.05%), and 10 with poor BG control and mild to moderate diabetic ketoacidosis (DKA) (HbA1C 9.12 +/-2.25%, strongly positive ketonuria 3+ and elevated plasma beta-hydroxybutyrate). Erythrocyte membrane fluidity was determined by fluorescence polarization using 6-(9-anthroyloxy stearic acid as fluorescent probe.
Membrane fluidity was normal in the diabetic patients with good BG control but significantly lower in the two groups of patients with poor BG control than in the healthy subjects (P < 0.01). The membrane fluidity in the poor BG control groups was also lower in the patients with DKA than in those without DKA (P < 0.01).
The factors that most influence membrane fluidity in IDDM patients appear to be hyperglycemia and ketone bodies.
研究可能改变胰岛素依赖型糖尿病（IDDM）患者红细胞膜流动性的因素。
受试者包括10名健康男性和30名IDDM患者，其中10名血糖（BG）控制良好（糖化血红蛋白[HbA1c]为5.88±0.60%[均值±标准差]），10名BG控制不佳（HbA1C为9.48±1.05%），10名BG控制不佳且伴有轻至中度糖尿病酮症酸中毒（DKA）（HbA1C为9.12±2.25%，尿酮体强阳性3+，血浆β-羟基丁酸升高）。以6-（9-蒽氧基）硬脂酸作为荧光探针，采用荧光偏振法测定红细胞膜流动性。
BG控制良好的糖尿病患者膜流动性正常，但BG控制不佳的两组患者的膜流动性显著低于健康受试者（P<0.01）。BG控制不佳组中，伴有DKA的患者的膜流动性也低于不伴有DKA的患者（P<0.01）。
IDDM患者中，对膜流动性影响最大的因素似乎是高血糖和酮体。
