Hutchinson T H, Jha A N, Dixon D R
Brixham Environmental Laboratory, ZENECA Limited, Freshwater Quarry, Brixham, Devon, United Kingdom.
Ecotoxicol Environ Saf. 1995 Aug;31(3):271-81. doi: 10.1006/eesa.1995.1074.
The polychaete Platynereis dumerilii (Polychaeta: Nereidae) has been evaluated as a candidate bioassay species for marine ecotoxicity testing. The species conforms with many of the requirements of an ideal bioassay organism in that (i) it is amenable to laboratory culture, (ii) its relatively small size makes it convenient for handling and laboratory exposure studies, (iii) its diet is defined and can be controlled, (iv) it reproduces throughout the year and, using photoperiod manipulation, can be induced to spawn as required, and (v) it has a short life cycle (approximately 3 months at 20 degrees C) making it feasible to study the effects of xenobiotics on chronic endpoints such as reproduction. The components of the life history which have been examined to date include fertilization rate, embryo-larval development, and larval survival. These life stages were evaluated using the reference materials used in the 1991 International Paris Commission (PARCOM) Ring Test (namely, the biocides, Bioban P-1487 and Vantocil IB, and the widely used reference toxicant, 3,5-dichlorophenol). For fertilization rate, the median effect concentrations (1-h EC50 values) were 0.32 mg.liter-1 for Bioban P-1487, 1.92 [corrected] mg.liter-1 for 3,5-dichlorophenol, and 9.66 mg.liter-1 for Vantocil IB. For embryo-larval development, the median effect concentrations (48-h EC50 values) were 0.29 mg.liter-1 for Bioban P-1487, 2.13 mg.liter-1 for 3,5-dichlorophenol, and 4.81 mg.liter-1 for Vantocil IB. For larval survival, the median lethal concentrations (48-h LC50 values) were 0.32 mg.liter-1 for Bioban P-1487, 3.64 mg.liter-1 for 3,5-dichlorophenol, and 10.9 mg.liter-1 for Vantocil IB. These results (all based on nominal values) suggest that, for these reference materials, the early life stages of P. dumerilii are of similar sensitivity to other marine invertebrate species. Together with the amenability of this species to laboratory culture, these data suggest that P. dumerilii has significant potential for use in marine ecotoxicity testing.
多毛纲动物杜氏阔沙蚕（Polychaeta: Nereidae）已被评估为海洋生态毒性测试的候选生物测定物种。该物种符合理想生物测定生物的许多要求，即：（i）适合实验室培养；（ii）相对较小的体型便于处理和进行实验室暴露研究；（iii）其食物种类明确且可控制；（iv）全年均可繁殖，通过光周期调控，可根据需要诱导产卵；（v）生命周期较短（20摄氏度下约3个月），使得研究异生素对诸如繁殖等慢性终点的影响成为可能。迄今为止已研究的生活史组成部分包括受精率、胚胎 - 幼虫发育和幼虫存活率。使用1991年国际巴黎委员会（PARCOM）环形测试中使用的参考物质（即杀生物剂Bioban P - 1487和Vantocil IB，以及广泛使用的参考毒物3,5 - 二氯苯酚）对这些生活阶段进行了评估。对于受精率，Bioban P - 1487的半数效应浓度（1小时EC50值）为0.32毫克/升，3,5 - 二氯苯酚为1.92[校正后]毫克/升，Vantocil IB为9.66毫克/升。对于胚胎 - 幼虫发育，Bioban P - 1487的半数效应浓度（48小时EC50值）为0.29毫克/升，3,5 - 二氯苯酚为2.13毫克/升，Vantocil IB为4.81毫克/升。对于幼虫存活率，Bioban P - 1487的半数致死浓度（48小时LC50值）为0.32毫克/升，3,5 - 二氯苯酚为3.64毫克/升，Vantocil IB为10.9毫克/升。这些结果（均基于标称值）表明，对于这些参考物质，杜氏阔沙蚕的早期生活阶段与其他海洋无脊椎动物物种具有相似的敏感性。结合该物种适合实验室培养这一特性，这些数据表明杜氏阔沙蚕在海洋生态毒性测试中具有巨大的应用潜力。
