Seki N, Moczko M, Nagase T, Zufall N, Ehmann B, Dietmeier K, Schäfer E, Nomura N, Pfanner N
Kazusa DNA Research Institute, Chiba, Japan.
FEBS Lett. 1995 Nov 20;375(3):307-10. doi: 10.1016/0014-5793(95)01229-8.
Import of preproteins into mitochondria requires transport machineries in both mitochondrial membranes that have been characterized in Saccharomyces cerevisiae and Neurospora crassa. By cDNA analysis, we identified a human protein of 16 kDa with significant overall homology to the fungal mitochondrial import receptor Mom19, including the three typical characteristics: a hydrophobic N-terminal segment, a tetratrico peptide motif in the middle and a negatively charged C-terminus. The human Mom19 homolog is expressed in all tissues analyzed. When synthesized in vitro, the human Mom19 homolog is targeted to isolated yeast mitochondria and specifically associates with the outer membrane receptor complex, suggesting that indeed a mitochondrial import receptor was identified.
前体蛋白导入线粒体需要线粒体内外膜上的转运机制，这在酿酒酵母和粗糙脉孢菌中已有研究。通过cDNA分析，我们鉴定出一种16kDa的人类蛋白，它与真菌线粒体导入受体Mom19具有显著的整体同源性，包括三个典型特征：疏水的N端片段、中间的四三肽基序和带负电荷的C端。人类Mom19同源物在所有分析的组织中均有表达。在体外合成时，人类Mom19同源物靶向分离的酵母线粒体，并特异性地与外膜受体复合物结合，这表明确实鉴定出了一种线粒体导入受体。
