宿主和噬菌体X 174的突变影响50S复合物的形态发生或稳定性，50S复合物是一种单链DNA合成中间体。
Host and phi X 174 mutations affecting the morphogenesis or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediate.
作者信息
Ekechukwu M C, Oberste D J, Fane B A
机构信息
Department of Biological Sciences, University of Arkansas, Fayetteville 72701, USA.
出版信息
Genetics. 1995 Aug;140(4):1167-74. doi: 10.1093/genetics/140.4.1167.
Abstract
The morphogenetic pathway of bacteriophage phi X 174 was investigated in rep mutant hosts that specifically block stage III single-stranded DNA synthesis. The defects conferred by the mutant rep protein most likely affect the formation or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediate, which consists of a viral prohead and a DNA replicating intermediate (preinitiation complex). phi X 174 mutants, ogr (rep), which restore the ability to propagate in the mutant rep hosts, were isolated. The org (rep) mutations confer amino acid substitutions in the viral coat protein, a constituent of the prohead, and the viral A protein, a constituent of the preinitiation complex. Four of the six coat protein substitutions are localized on or near the twofold axis of symmetry in the atomic structure of the mature virion.
摘要
在特异性阻断III期单链DNA合成的rep突变宿主中研究了噬菌体phi X 174的形态发生途径。突变的rep蛋白造成的缺陷很可能影响50S复合物的形成或稳定性，50S复合物是一种单链DNA合成中间体，由病毒原头部和DNA复制中间体（预起始复合物）组成。分离出了phi X 174突变体ogr（rep），其恢复了在突变rep宿主中繁殖的能力。ogr（rep）突变导致病毒衣壳蛋白（原头部的一个组成部分）和病毒A蛋白（预起始复合物的一个组成部分）中的氨基酸替换。六个衣壳蛋白替换中有四个位于成熟病毒粒子原子结构中二重对称轴上或其附近。
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