Giordano R, O'Neill S L, Robertson H M
Department of Entomology, University of Illinois, Urbana 61801, USA.
Genetics. 1995 Aug;140(4):1307-17. doi: 10.1093/genetics/140.4.1307.
Various stocks of Drosophila mauritiana and D. sechellia were found to be infected with Wolbachia, a Rickettsia-like bacterium that is known to cause cytoplasmic incompatibility and other reproductive abnormalities in arthropods. Testing for the expression of cytoplasmic incompatibility in these two species showed partial incompatibility in D. sechellia but no expression of incompatibility in D. mauritiana. To determine whether absence of cytoplasmic incompatibility in D. mauritiana was due to either the bacterial or host genome, we transferred bacteria from D. mauritiana into an uninfected strain of D. simulans, a host species known to express high levels of incompatibility with endogenous Wolbachia. We also performed the reciprocal transfer of the natural D. simulans Riverside infection into a tetracycline-treated stock of D. mauritiana. In each case, the ability to express incompatibility was unaltered by the different host genetic background. These experiments indicate that in D. simulans and D. mauritiana expression of the cytoplasmic incompatibility phenotype is determined by the bacterial strain and that D. mauritiana harbors a neutral strain of Wolbachia.
研究发现，多种毛里求斯果蝇和塞舌尔果蝇种群感染了沃尔巴克氏体，这是一种类似立克次氏体的细菌，已知会在节肢动物中导致细胞质不亲和及其他生殖异常。对这两个物种的细胞质不亲和表达进行检测发现，塞舌尔果蝇存在部分不亲和现象，而毛里求斯果蝇未表现出不亲和。为确定毛里求斯果蝇不存在细胞质不亲和是由于细菌基因组还是宿主基因组的原因，我们将毛里求斯果蝇体内的细菌转移到未感染的拟暗果蝇品系中，拟暗果蝇是一种已知会与内源性沃尔巴克氏体表现出高度不亲和的宿主物种。我们还将天然的拟暗果蝇河滨感染株进行反向转移，导入经四环素处理的毛里求斯果蝇种群中。在每种情况下，不同宿主遗传背景均未改变表达不亲和的能力。这些实验表明，在拟暗果蝇和毛里求斯果蝇中，细胞质不亲和表型的表达由细菌菌株决定，且毛里求斯果蝇携带一种中性的沃尔巴克氏体菌株。
