胸腺瘤表达与兰尼碱受体共有的表位。

Thymomas express epitopes shared by the ryanodine receptor.

作者信息

Mygland A, Kuwajima G, Mikoshiba K, Tysnes O B, Aarli J A, Gilhus N E

机构信息

Department of Neurology, University of Bergen, Norway.

出版信息

J Neuroimmunol. 1995 Oct;62(1):79-83. doi: 10.1016/0165-5728(95)00106-c.

DOI:10.1016/0165-5728(95)00106-c

PMID:7499495

Abstract

Myasthenia gravis (MG) patients with thymoma have antibodies against ryanodine receptor (RyR) of skeletal and heart muscle. In this study, thymomas were examined for reactivity with a panel of polyclonal rabbit antibodies against various short peptides of RyR. An antibody against peptide C2 in the transmembrane region of RyR stained thymoma epithelial cells in cryosections of 17/23 thymomas, and detected a 40-kDa peptide in Western blotting of a thymoma membrane fraction. The other RyR antibodies did not react with thymoma tissue. The anti-C2 RyR antibody did not react with normal thymus, tonsil or carcinoma of colon. The results strongly indicate that epithelial thymoma cells express an epitope shared by the transmembrane region of skeletal and cardiac muscle RyR.

摘要

患有胸腺瘤的重症肌无力（MG）患者体内存在针对骨骼肌和心肌兰尼碱受体（RyR）的抗体。在本研究中，使用一组针对RyR各种短肽的多克隆兔抗体检测胸腺瘤的反应性。一种针对RyR跨膜区域肽C2的抗体在23例胸腺瘤中的17例冰冻切片中对胸腺瘤上皮细胞进行了染色，并在胸腺瘤膜部分的蛋白质印迹中检测到一条40 kDa的肽。其他RyR抗体未与胸腺瘤组织发生反应。抗C2 RyR抗体不与正常胸腺、扁桃体或结肠癌发生反应。结果强烈表明，上皮性胸腺瘤细胞表达骨骼肌和心肌RyR跨膜区域共有的一个表位。