Sapatino B V, Petrescu A D, Rosenbaum B A, Smith R, Piedrahita J A, Welsh C J
Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station 77843-4458, USA.
J Neuroimmunol. 1995 Nov;62(2):127-35. doi: 10.1016/0165-5728(95)00094-4.
Cloned cerebrovascular endothelial cells (CVE) persistently infected with Theiler's virus (PI-CVE) have been established and characterized. The CVE were derived from strains of mice that are susceptible (SJL/J and CBA) and resistant (BALB/c) to Theiler's virus-induced demyelination (TVID). The cells were persistently infected with either the BeAn or GDVII strains of Theiler's virus in vitro and studied at various passage levels for infectious virus, viral antigen and the expression of major histocompatibility complex (MHC) Class I and II antigens. The virus replicated to lower titers than in acutely infected CVE and appeared to be more cell-associated. Flow cytometric analysis revealed that 18-39% of the PI-CVE contained viral antigen. Persistently infected CVE derived from SJL/J and CBA mice expressed high levels of MHC Class I, whereas BALB/c PI-CVE did not. MHC Class II was upregulated by IFN-gamma in SJL/J PI-CVE albeit at a slightly lower level than in uninfected CVE. In addition, the PI-CVE demonstrated increased levels of mRNA for IL-1 beta when compared to uninfected CVE.
已建立并鉴定了持续感染泰勒氏病毒的克隆脑血管内皮细胞（CVE，PI-CVE）。CVE源自对泰勒氏病毒诱导的脱髓鞘（TVID）敏感（SJL/J和CBA）和抗性（BALB/c）的小鼠品系。这些细胞在体外被泰勒氏病毒的BeAn或GDVII株持续感染，并在不同传代水平上研究感染性病毒、病毒抗原以及主要组织相容性复合体（MHC）I类和II类抗原的表达。该病毒复制的滴度低于急性感染的CVE，且似乎与细胞的关联性更强。流式细胞术分析显示，18%-39%的PI-CVE含有病毒抗原。源自SJL/J和CBA小鼠的持续感染CVE表达高水平的MHC I类，而BALB/c PI-CVE则不表达。在SJL/J PI-CVE中，MHC II类被干扰素-γ上调，尽管水平略低于未感染的CVE。此外，与未感染的CVE相比，PI-CVE的IL-1β mRNA水平有所升高。
