Kuramoto H, Kuwano R
Department of Anatomy, Yamanashi Medical University, Japan.
J Auton Nerv Syst. 1995 Aug 4;54(2):126-36. doi: 10.1016/0165-1838(95)00004-h.
To determine the origin of the calbindin-containing laminar nerve endings in the myenteric ganglia of the rat esophagus, retrograde tracing experiments combined with immunohistochemistry using an antibody for calbindin were carried out. After Fast blue was injected into the cervical portion of the esophagus, labeled neurons were found bilaterally in the nodose ganglion and dorsal root ganglia of C1 to T3. 80% of the total neurons in the nodose ganglion and 20% of those in the dorsal root ganglia showed calbindin immunoreactivity. Moreover, 79% of Fast-blue-labeled neurons found in the nodose ganglion and 18% of those in the dorsal root ganglia were immunoreactive for calbindin. These results suggest that the calbindin antibody we used is useful as a marker for identifying esophageal vagal afferents derived from the nodose ganglion. The calbindin-immunoreactive nerve fibers forming the laminar endings in the myenteric ganglia of the rat cervical esophagus are mainly derived from sensory neurons in the nodose ganglion and partly derived from those in the cervical and upper thoracic dorsal root ganglia. Calbindin-containing laminar nerve endings may be related to mechanoreceptors in the esophagus.
为了确定大鼠食管肌间神经节中含钙结合蛋白的层状神经末梢的起源，我们进行了逆行追踪实验，并结合使用钙结合蛋白抗体的免疫组织化学方法。将快蓝注入食管颈部后，在双侧的结状神经节以及C1至T3的背根神经节中发现了标记神经元。结状神经节中80%的神经元以及背根神经节中20%的神经元显示出钙结合蛋白免疫反应性。此外，在结状神经节中发现的79%的快蓝标记神经元以及背根神经节中18%的快蓝标记神经元对钙结合蛋白呈免疫反应性。这些结果表明，我们使用的钙结合蛋白抗体可作为识别源自结状神经节的食管迷走传入神经的标志物。在大鼠颈段食管肌间神经节中形成层状末梢的钙结合蛋白免疫反应性神经纤维主要源自结状神经节中的感觉神经元，部分源自颈段和上胸段背根神经节中的感觉神经元。含钙结合蛋白的层状神经末梢可能与食管中的机械感受器有关。
