Kosmehl H, Berndt A, Katenkamp D, Hyckel P, Stiller K J, Gabler U, Langbein L, Reh T
Institute of Pathology, Friedrich Schiller University of Jena, Germany.
J Oral Pathol Med. 1995 Sep;24(8):343-8. doi: 10.1111/j.1600-0714.1995.tb01197.x.
The expression of extracellular matrix (ECM) proteins (fibronectin, laminin, collagen IV) and ECM receptors of integrin type (alpha 2 beta 1, collagen receptor; alpha 6 chain of the fibronectin receptor; alpha 6 chain of the laminin receptor) were examined in normal oral squamous epithelium and in invasive areas of squamous cell carcinomas with various differentiation and proliferation activities (Ki-67 antigen labelling), evaluating the presence, quantity (using an image analysis system) and distribution of the integrin subunits. In the mucosa, there was uniform immunostaining for alpha 2 beta 1 and alpha 6 concentrated at the cell membrane in the basal/supra basal cell zone, whereas, alpha 5 showed a discontinuous staining of the basal cell-basement membrane interface. alpha 2 and alpha 6 could be visualized in all carcinomas. alpha 5 showed low expression preferentially in less differentiated carcinomas. In contrast to normal mucosa, there was an increase in alpha 6 staining in well-differentiated carcinomas. Dedifferentiation of oral carcinomas was accompanied by an increase in cellular proliferation and with a decrease in alpha 2 beta 1 and alpha 6 staining. This reduction of alpha 6 staining was shown to be statistically significant, suggesting that this integrin may be a valuable grading parameter for oral squamous cell carcinoma.
在正常口腔鳞状上皮以及具有不同分化和增殖活性（Ki-67抗原标记）的鳞状细胞癌浸润区域，检测细胞外基质（ECM）蛋白（纤连蛋白、层粘连蛋白、IV型胶原）和整合素型ECM受体（α2β1，胶原受体；纤连蛋白受体的α6链；层粘连蛋白受体的α6链）的表达，评估整合素亚基的存在、数量（使用图像分析系统）及分布。在黏膜中，α2β1和α6呈现均匀免疫染色，集中于基底/基底上层细胞区的细胞膜，而α5在基底细胞-基底膜界面呈间断染色。α2和α6在所有癌组织中均可观察到。α5在低分化癌中优先呈现低表达。与正常黏膜相反，高分化癌中α6染色增加。口腔癌的去分化伴随着细胞增殖增加以及α2β1和α6染色减少。α6染色的这种减少具有统计学意义，提示该整合素可能是口腔鳞状细胞癌的一个有价值的分级参数。
