Danahey D G, Wu J C, Lin L H, DePhilip R M
Department of Cell Biology, Ohio State University, Columbus 43210, USA.
J Urol. 1995 Dec;154(6):2190-6.
We describe a monoclonal antibody (Mab B2) against vimentin filaments in rat Sertoli cells and in a subset of epithelial cells in the rat epididymis, prostate and urinary bladder.
The immunoreactivity of Mab B2 was examined on cryostat sections and immunoblots and compared with the reactivity of a previously characterized monoclonal antibody against vimentin.
While both antibodies recognized vimentin in Sertoli cells, only Mab B2 recognized vimentin in urogenital tract epithelia.
Vimentin is expressed in the urogenital tract, and differential reactivities of antibodies may be responsible for conflicting results reported elsewhere for vimentin expression.
我们描述了一种针对大鼠支持细胞以及大鼠附睾、前列腺和膀胱中一部分上皮细胞波形蛋白丝的单克隆抗体（单克隆抗体B2）。
在低温恒温器切片和免疫印迹上检测单克隆抗体B2的免疫反应性，并与先前鉴定的一种针对波形蛋白的单克隆抗体的反应性进行比较。
虽然两种抗体都能识别支持细胞中的波形蛋白，但只有单克隆抗体B2能识别泌尿生殖道上皮中的波形蛋白。
波形蛋白在泌尿生殖道中表达，抗体的不同反应性可能是其他地方报道的关于波形蛋白表达的相互矛盾结果的原因。
