Olah I, Kendall C, Glick B
Poultry Science Department, Clemson University, South Carolina 29634-0379.
Anat Rec. 1992 May;233(1):111-20. doi: 10.1002/ar.1092330115.
Embryonic and posthatched differentiation of bursal secretory dendritic cells, which express vimentin intermediate filaments, were studied with anti-vimentin (clone 3B4) and anti-cytokeratin (clone Lu5) monoclonal antibodies. Anti-cytokeratin staining revealed that medullary reticular epithelial cells formed a continuous network at every age, whereas the vimentin positive cells were single and showed dendritic appearance. On the basis of location, number, shape, polarized appearance, and Ia staining, the vimentin-positive cells and secretory dendritic cells appeared to be the same cell. Secretory dendritic cell precursors entered the bursal epithelium between 11 and 13 days of embryogenesis. The first vimentin positive cell appeared in the bud of 14-day embryos. Bud formation preceded the appearance of vimentin-positive cells. These observations suggested that the secretory dendritic cell precursor did not express vimentin when it entered the epithelium. Between 15 days of embryogenesis and 2 weeks of posthatch development, the changes in vimentin staining pattern revealed a cytological differentiation of the vimentin-positive cell. During rapid bursal growth, the number of secretory dendritic cells (vimentin-positive cells) increased about 18 times possibly by proliferation of vimentin-negative precursors in the epithelial arches of the corticomedullary border.
运用抗波形蛋白（克隆号3B4）和抗细胞角蛋白（克隆号Lu5）单克隆抗体，对表达波形蛋白中间丝的法氏囊分泌性树突状细胞在胚胎期及出壳后的分化进行了研究。抗细胞角蛋白染色显示，髓质网状上皮细胞在各个年龄段均形成连续的网络，而波形蛋白阳性细胞为单个，呈树突状外观。基于位置、数量、形状、极化外观和Ia染色，波形蛋白阳性细胞与分泌性树突状细胞似乎是同一类细胞。分泌性树突状细胞前体在胚胎发育的第11至13天进入法氏囊上皮。第一个波形蛋白阳性细胞出现在14天胚胎的芽中。芽的形成先于波形蛋白阳性细胞的出现。这些观察结果表明，分泌性树突状细胞前体进入上皮时不表达波形蛋白。在胚胎发育的第15天至出壳后2周的发育过程中，波形蛋白染色模式的变化揭示了波形蛋白阳性细胞的细胞学分化。在法氏囊快速生长期间，分泌性树突状细胞（波形蛋白阳性细胞）的数量可能通过皮质髓质交界处上皮弓中波形蛋白阴性前体的增殖而增加了约18倍。
