Kemp P M, Abukhalaf I K, Manno J E, Manno B R, Alford D D, Abusada G A
Louisiana State University, Department of Pharmacology, USA.
J Anal Toxicol. 1995 Sep;19(5):285-91. doi: 10.1093/jat/19.5.285.
This report describes a method for the quantitative analysis of delta 9-tetrahydrocannabinol and six of its metabolites, 8 alpha-hydroxy-delta 9-tetrahydrocannabinol, 8 beta-hydroxy-delta 9-tetrahydrocannabinol, 11-hydroxy-delta 9-tetrahydrocannabinol, 8 alpha,11-dihydroxy-delta 9-tetrahydrocannabinol, 8 beta,11-dihydroxy-delta 9-tetrahydrocannabinol, and 11-nor-9-carboxy-delta 9-tetrahydrocannabinol. In addition, the method was designed to detect cannabidiol and cannabinol, two naturally occurring cannabinoids. Plasma and urine samples were hydrolyzed with bacterial (Escherichia coli) beta-glucuronidase and extracted with hexane-ethyl acetate (7:1). Analysis and quantitation were performed by gas chromatography-mass spectrometry in the electron ionization mode coupled with selected ion monitoring. The cannabinoids were detected as their trimethylsilyl derivatives to enhance their chromatographic separation and mass spectral characteristics. The linearity of the procedure was excellent for all of the compounds within the range tested (0-100 ng/mL). Limits of detection ranged from 0.5 to 1.5 ng/mL in urine and from 0.6 to 2.1 ng/mL in plasma depending on the analyte.
本报告描述了一种对Δ⁹-四氢大麻酚及其六种代谢物进行定量分析的方法，这六种代谢物分别为8α-羟基-Δ⁹-四氢大麻酚、8β-羟基-Δ⁹-四氢大麻酚、11-羟基-Δ⁹-四氢大麻酚、8α,11-二羟基-Δ⁹-四氢大麻酚、8β,11-二羟基-Δ⁹-四氢大麻酚和11-去甲-9-羧基-Δ⁹-四氢大麻酚。此外，该方法还用于检测大麻二酚和大麻酚这两种天然存在的大麻素。血浆和尿液样本用细菌（大肠杆菌）β-葡萄糖醛酸酶进行水解，并用己烷 - 乙酸乙酯（7:1）进行萃取。通过气相色谱 - 质谱联用仪在电子电离模式下结合选择离子监测进行分析和定量。大麻素以其三甲基硅烷基衍生物的形式被检测，以增强其色谱分离效果和质谱特征。在所测试的范围内（0 - 100 ng/mL），该方法对所有化合物的线性关系都非常好。根据分析物的不同，尿液中的检测限范围为0.5至1.5 ng/mL，血浆中的检测限范围为0.6至2.1 ng/mL。
