Department of Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia.
Molecules. 2020 Dec 11;25(24):5872. doi: 10.3390/molecules25245872.
An original gas chromatographic method has been developed for simultaneous determination of major terpenes and cannabinoids in plant samples and their extracts. The main issues to be addressed were the large differences in polarity and volatility between both groups of analytes, but also the need for an exhaustive decarboxylation of cannabinoid acidic forms. Sample preparation was minimised, also by avoiding any analyte derivatisation. Acetone was found to be the most appropriate extraction solvent. Successful chromatographic separation was achieved by using a medium polarity column. Limits of detection ranged from 120 to 260 ng/mL for terpenes and from 660 to 860 ng/mL for cannabinoids. Parallel testing proved the results for cannabinoids are comparable to those obtained from established HPLC methods. Despite very large differences in concentrations between both analyte groups, a linear range between 1 and 100 µg/mL for terpenes and between 10 and 1500 µg/mL for cannabinoids was determined.
已经开发出一种原始的气相色谱方法，用于同时测定植物样品及其提取物中的主要萜类化合物和大麻素。需要解决的主要问题是这两组分析物之间在极性和挥发性方面存在很大差异，但也需要对大麻素的酸性形式进行彻底脱羧。通过避免任何分析物衍生化，最小化了样品制备。发现丙酮是最合适的提取溶剂。通过使用中等极性柱实现了成功的色谱分离。萜类化合物的检测限范围为 120 至 260ng/mL，而大麻素的检测限范围为 660 至 860ng/mL。平行测试证明，大麻素的结果与从已建立的 HPLC 方法获得的结果相当。尽管两组分析物之间的浓度差异非常大，但确定了萜类化合物在 1 至 100µg/mL 之间和大麻素在 10 至 1500µg/mL 之间的线性范围。
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