Kintz P, Machart D, Jamey C, Mangin P
Institut de Medecine Legale, Strasbourg, France.
J Anal Toxicol. 1995 Sep;19(5):304-6. doi: 10.1093/jat/19.5.304.
Gas chromatography-mass spectrometry (GC-MS) and immunological methods, including the Syva enzyme multiplied immunoassay technique, the Abbott fluorescence polarization immunoassay, and the Roche OnLine immunoassay, were compared for the determination of 11-nor-delta 9-tetrahydrocannabinol-9- carboxylic acid (THCCOOH). The results of all three immunoassays were not in accordance with the GC-MS results in three cases of a 72-specimen panel. Only one false negative was observed using the OnLine immunoassay. The immunological methods compared favorably and are acceptable for detecting the presence of cannabis metabolites in urine. These results support the concept that all immunoassays for cannabinoids should be considered as screening procedures. No concentration correlation between GC-MS and the immunoassays could be established because of the different cross-reactivities of the metabolites.
比较了气相色谱 - 质谱联用（GC-MS）和免疫分析方法，包括西瓦酶放大免疫分析技术、雅培荧光偏振免疫分析以及罗氏在线免疫分析，用于测定11-去甲-δ⁹-四氢大麻酚-9-羧酸（THCCOOH）。在一个包含72个样本的检测组中，有3例样本的三种免疫分析结果均与GC-MS结果不一致。使用在线免疫分析仅观察到1例假阴性。所比较的免疫分析方法表现良好，可用于检测尿液中大麻代谢物的存在。这些结果支持这样一种观点，即所有大麻素免疫分析都应被视为筛查程序。由于代谢物的交叉反应性不同，无法建立GC-MS与免疫分析之间的浓度相关性。
