Armbruster D A, Schwarzhoff R H, Hubster E C, Liserio M K
Human System Center (AFMC), Armstrong Laboratory, Brooks AFB, TX 78235-5240.
Clin Chem. 1993 Oct;39(10):2137-46.
The newest formulation of the Syva EMIT assay for drugs of abuse, EMIT II, and a new immunoassay, OnLine (Roche), utilizing the kinetic interaction of microparticles in solution (KIMS) methodology, RIA tests, and TDx fluorescence polarization immunoassay (FPIA) procedures were compared for marijuana, cocaine, opiates, and barbiturates. Both EMIT II and OnLine immunoassays were performed with a Hitachi 717 analyzer. Calibration curves, the degree of separation between negative and cutoff calibrators, precision, likelihood of carryover from positive to negative samples, and overall ease and speed of analysis were evaluated. RIA and OnLine detected 99% of gas chromatography/mass spectrometry (GC/MS)-confirmed marijuana samples; TDx, 95%; and EMIT II, 88%. All four immunoassays detected approximately 99% of confirmed cocaine-positive urines. RIA, OnLine, and TDx all detected 100% of opiate-confirmed samples; EMIT II, 97%. Barbiturate assays exhibited the greatest disparity, with OnLine and TDx detecting 100% of confirmed positives; EMIT II, 88%; and RIA, 78%. For a variety of reasons, we prefer the fully automated EMIT II and OnLine assays for high-volume urine testing, in comparison with our laboratory's semiautomated RIA tests and the limited-throughput TDx system. The four immunoassays investigated delivered comparable performance in terms of detection rates for GC/MS-confirmed positives for some drugs but not for others.
对滥用药物的Syva EMIT检测法的最新版本EMIT II、一种利用溶液中微粒动力学相互作用（KIMS）方法的新型免疫测定法OnLine（罗氏公司）、放射免疫分析（RIA）测试以及TDx荧光偏振免疫分析（FPIA）程序进行了大麻、可卡因、阿片类药物和巴比妥类药物检测方面的比较。EMIT II和OnLine免疫测定均使用日立717分析仪进行。评估了校准曲线、阴性和临界校准品之间的分离程度、精密度、从阳性样品到阴性样品的携带污染可能性以及整体分析的简易程度和速度。RIA和OnLine检测出99%经气相色谱/质谱（GC/MS）确认的大麻样品；TDx为95%；EMIT II为88%。所有四种免疫测定法检测出约99%经确认的可卡因阳性尿液。RIA、OnLine和TDx均检测出100%经确认的阿片类药物样品；EMIT II为97%。巴比妥类药物检测的差异最大，OnLine和TDx检测出100%经确认的阳性样品；EMIT II为88%；RIA为78%。出于多种原因，与我们实验室的半自动RIA测试和通量有限的TDx系统相比，我们更倾向于使用全自动的EMIT II和OnLine测定法进行大批量尿液检测。所研究的四种免疫测定法在某些药物经GC/MS确认的阳性样品检测率方面表现相当，但在其他药物方面并非如此。
